Disease relevance of NSC9866

• The analogue 4-acetylaminofluorene (4-AAF) was much less effective than 2-AAF as a promoter of large nodules and carcinomas, but promoted the outgrowth of medium-sized nodules (1 < 2.5 mm) [1].
• Of the aromatic amines tested, 2-aminofluorene and, with lower efficiency, 2-acetylaminofluorene were photoactivated, whereas irradiation of 4-acetylaminofluorene and of the 1- and 2-amino substitutes of anthracene and naphthalene did not produce mutagenic derivatives in Salmonella typhimurium [2].

High impact information on NSC9866

• In contrast, 4-acetylaminofluorene, an isomer of 2-AAF, and trans-4-acetylaminostilbene do not bind to the rat hepatic cytosolic Ah receptor [3].
• 4-Acetylaminofluorene and 2-amino-3-methylimidazo[4,5-f]quinoline did not induce sister chromatid exchanges in the investigated cell lines [4].
• A series of experiments comparing MCP with the potent mitogen 4-acetylaminofluorene (4AAF) and with 2/3 partial hepatectomy showed unexpected differences between the three treatment regimes as monitored by the expression of micronucleated hepatocytes (MH) in livers initiated with N-nitrosodimethylamine (NDMA) [5].
• Administration of 4-acetylaminofluorene (4AAF) to rats by oral gavage (1000 mg/kg) produces a wave of S-phase activity in the liver 36 h later, followed by a wave of mitoses at 48 h [6].
• The present studies were undertaken to provide a rationale for the observation that benzo(a)pyrene and 2-acetylaminofluorene induce the hepatic CYP1A1 protein, whereas their non-carcinogenic isomers benzo(e)pyrene and 4-acetylaminofluorene are, at best, relatively very weak inducers [7].

Biological context of NSC9866

• A strong positive response was observed in the cell proliferation assay for the test agent and the positive control agents quinoline and 4-acetylaminofluorene [8].
• Cytoskeleton modifications induced by phenobarbital, 2-acetylaminofluorene and 4-acetylaminofluorene in normal and initiated/selected hepatocytes: relation with the "resistant" phenotype [9].

Anatomical context of NSC9866

• Lack of DNA single-strand breaks in rat liver cells exposed to 4-acetylaminofluorene, in vivo and in vitro [10].
• The influence of chemical carcinogens on natural killer cell function in rats. A comparison of 2-acetylaminofluorene with 4-acetylaminofluorene [11].

Gene context of NSC9866

• Molecular modelling of cytochrome CYP1A1: a putative access channel explains differences in induction potency between the isomers benzo(a)pyrene and benzo(e)pyrene, and 2- and 4-acetylaminofluorene [7].
• The induction of ornithine decarboxylase (ODC) in rat liver by the carcinogen 2-acetylaminofluorene (2AAF), the strong liver tumor initiator trans-4-acetylaminostilbene (AAS), the non-carcinogen 4-acetylaminofluorene (4AAF) and, as a control, 3-methylcholanthrene (MC) has been examined [12].

Analytical, diagnostic and therapeutic context of NSC9866

• The potent rat-liver mitogen 4-acetylaminofluorene (4AAF) is shown here to provide an effective replacement for the surgical procedure of 2/3 partial hepatectomy (2/3PH) in the in vivo rat-liver micronucleus assay described by Tates and his colleagues [13].

References