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Gene Review

ompA  -  outer membrane protein A

Escherichia coli CFT073

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Disease relevance of ompA

  • Highly purified RNAase E correctly processed E. coli 5S ribosomal RNA, bacteriophage T4 gene 32 mRNA and E. coli ompA mRNA at sites known to depend on the rne gene for cleavage in vivo [1].
  • MicA is conserved in many enterobacteria, as is its ompA target site [2].
  • Bacteriophage sensitivity testing indicated an association between bacteriophage resistance and isolates having the novel ompA allele [3].
  • The ompA genes of ADP1 and an additional strain, Acinetobacter sp. V-26 were cloned and sequenced [4].
  • The ompA genes encoding the 40 kDa major outer membrane protein (MOMP) of Chlamydophila (Ch.) abortus, Ch. pecorum, and Chlamydia (C.) suis were cloned into the arabinose-inducible plasmid vector pBADMycHis, and recombinant MOMPs (rMOMP) from the three chlamydial species were expressed at high levels in Escherichia (E.) coli [5].

High impact information on ompA

  • MicA binding generates a footprint across the ompA Shine-Dalgarno sequence, consistent with a 12 + 4 base-pair interaction, which is additionally supported by the effect of mutations in vivo and by bioinformatics analysis of enterobacterial micA/ompA homolog sequences [2].
  • Effects of ompA Deletion on Expression of Type 1 Fimbriae in Escherichia coli K1 Strain RS218 and on the Association of E. coli with Human Brain Microvascular Endothelial Cells [6].
  • Disruption of the ompA gene in the tdcA mutant strain abolished the hyperadherent phenotype, and anti-OmpA serum inhibited adhesion of wild-type and tdcA mutant strains to HeLa cells [7].
  • These findings imply that the ompA 5' UTR prolongs mRNA longevity by impeding multiple pathways for mRNA degradation [8].
  • Although close spacing of translating ribosomes is not critical for message stabilization by the ompA 5' UTR, mRNA longevity does require the periodic passage of ribosomes through the protein-coding region [8].

Biological context of ompA

  • This was 50% higher than the best result obtained from plasmid construct lacUV5par8cex, using the lacUV5 promoter and the ompA leader sequence [9].
  • Regulation of ompA by micA may have been retained due to a necessity for ompA control during one or both stages of the P. luminescens life cycle [10].
  • Furthermore, real-time PCR analysis indicates that the extent of polyadenylation of individual full-length transcripts such as lpp and ompA varies significantly in wild-type cells [11].

Analytical, diagnostic and therapeutic context of ompA


  1. Copurification of E. coli RNAase E and PNPase: evidence for a specific association between two enzymes important in RNA processing and degradation. Carpousis, A.J., Van Houwe, G., Ehretsmann, C., Krisch, H.M. Cell (1994) [Pubmed]
  2. Hfq-dependent regulation of OmpA synthesis is mediated by an antisense RNA. Udekwu, K.I., Darfeuille, F., Vogel, J., Reimegård, J., Holmqvist, E., Wagner, E.G. Genes Dev. (2005) [Pubmed]
  3. A Naturally Occurring Novel Allele of Escherichia coli Outer Membrane Protein A Reduces Sensitivity to Bacteriophage. Power, M.L., Ferrari, B.C., Littlefield-Wyer, J., Gordon, D.M., Slade, M.B., Veal, D.A. Appl. Environ. Microbiol. (2006) [Pubmed]
  4. The Acinetobacter outer membrane protein A (OmpA) is a secreted emulsifier. Walzer, G., Rosenberg, E., Ron, E.Z. Environ. Microbiol. (2006) [Pubmed]
  5. Expression of the major outer membrane protein (MOMP) of Chlamydophila abortus, Chlamydophila pecorum, and Chlamydia suis in Escherichia coli using an arabinose-inducible plasmid vector. Hoelzle, L.E., Hoelzle, K., Wittenbrink, M.M. J. Vet. Med. B Infect. Dis. Vet. Public Health (2003) [Pubmed]
  6. Effects of ompA Deletion on Expression of Type 1 Fimbriae in Escherichia coli K1 Strain RS218 and on the Association of E. coli with Human Brain Microvascular Endothelial Cells. Teng, C.H., Xie, Y., Shin, S., Di Cello, F., Paul-Satyaseela, M., Cai, M., Kim, K.S. Infect. Immun. (2006) [Pubmed]
  7. Multiple elements controlling adherence of enterohemorrhagic Escherichia coli O157:H7 to HeLa cells. Torres, A.G., Kaper, J.B. Infect. Immun. (2003) [Pubmed]
  8. mRNA stabilization by the ompA 5' untranslated region: two protective elements hinder distinct pathways for mRNA degradation. Arnold, T.E., Yu, J., Belasco, J.G. RNA (1998) [Pubmed]
  9. A two-stage refinement approach for the enhancement of excretory production of an exoglucanase from Escherichia coli. Fu, Z.B., Ng, K.L., Lam, C.C., Leung, K.C., Yip, W.H., Wong, W.K. Protein Expr. Purif. (2006) [Pubmed]
  10. Outer membrane protein genes and their small non-coding RNA regulator genes in Photorhabdus luminescens. Papamichail, D., Delihas, N. Biology direct [electronic resource]. (2006) [Pubmed]
  11. The majority of Escherichia coli mRNAs undergo post-transcriptional modification in exponentially growing cells. Mohanty, B.K., Kushner, S.R. Nucleic Acids Res. (2006) [Pubmed]
  12. Expression and purification of native human granulocyte-macrophage colony-stimulating factor from an Escherichia coli secretion vector. Libby, R.T., Braedt, G., Kronheim, S.R., March, C.J., Urdal, D.L., Chiaverotti, T.A., Tushinski, R.J., Mochizuki, D.Y., Hopp, T.P., Cosman, D. DNA (1987) [Pubmed]
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