Disease relevance of DDX20

• Gemin3: A novel DEAD box protein that interacts with SMN, the spinal muscular atrophy gene product, and is a component of gems [1].

High impact information on DDX20

• Gemin4 is associated in vivo with the SMN complex through a direct interaction with Gemin3 [2].
• In both compartments SMN is part of a large complex that contains several proteins including Gemin2 (formerly SIP1) and the DEAD box protein Gemin3 [2].
• Previous work has shown that DP103 binds viral proteins known to interact with a cellular transcription factor to modulate gene expression [3].
• DP103 is expressed predominantly in the testis and is also expressed at a lower level in other steroidogenic and nonsteroidogenic tissues [4].
• In addition, impaired transactivation of a DP103 binding-deficient EBNA2 mutant was rescued by overexpression of SMN [5].

Biological context of DDX20

• Here, we show that DP103 and SMN are complexed in B cells and that SMN coactivates the viral LMP promoter in the presence of EBNA2 in reporter gene assays and in vivo [5].
• Transcriptional factor FOXL2 interacts with DP103 and induces apoptosis [6].
• Overexpression of DP103 itself did not affect cell viability while its coexpression with FOXL2 led to the potentiation of cell death [6].

Anatomical context of DDX20

• Here we describe that in a human neuronal cell line, the miRNP proteins eIF2C2 (a member of the Argonaute family of proteins), Gemin3, and Gemin4 along with miRNAs cosediment with polyribosomes [7].
• Quantitative analysis of endogenous protein colocalization in growth cones after three-dimensional reconstructions revealed a statistically nonrandom association of SMN with Gemin2 (40%) and Gemin3 (48%) [8].
• Furthermore, a DP103 binding-deficient SMN mutant was released from nuclear gems and/or coiled bodies and further enhanced coactivation [5].
• Here, we show that FOXL2 induces apoptosis in both Chinese hamster ovary cells and rat granulosa cells, and it interacts with DP103, a DEAD box-containing protein [6].
• The repression is observed in five cell lines tested, while the degree of the repression varies greatly, which does not parallel with the expression level of the DEAD box protein of 130 kD (DP103), a potential interacting protein of a homologous domain in the steroidogenic factor 1 (SF-1) [9].

References