High impact information on Aurkb

• Finally, RKIP depletion or Raf hyperactivation reduces kinetochore localization and kinase activity of Aurora B, a regulator of the spindle checkpoint [1].
• AIM-1 message and protein accumulate at G2/M phase [2].
• Overexpression of kinase-inactive AIM-1 disrupts cleavage furrow formation without affecting nuclear division [2].
• At late anaphase, however, Aurora-B/AIM-1 is absent or mislocalized [3].
• Megakaryocytes treated with a proteasome inhibitor display Aurora-B/AIM-1 properly expressed and localized to the midzone, suggesting that protein degradation contributes to this atypical appearance [3].

Biological context of Aurkb

• Instead, kinase-inactive aurora B caused abnormal mitosis and deactivation of the spindle checkpoint [4].
• In contrast, inhibition of the kinase activity of aurora B appeared to have no effect on its interactions with centromeres or initial relocation onto midzone microtubules [4].
• Aurora B was found at centromeres at prophase and persisted until approximately 0.5 min after anaphase onset, when it redistributed to the spindle midzone and became concentrated at the equator along midzone microtubules [4].
• Depletion of Aurora-B/AIM-1 inhibited dissociation of HP1alpha from the chromosome arms at the G2-M transition [5].
• However, Aurora-B/AIM-1-mediated phosphorylation of H3 induced dissociation of the HP1alpha chromodomain but not of the intact protein in vitro, indicating that the center and/or C-terminal domain of HP1alpha interferes with the effect of H3 phosphorylation on HP1alpha dissociation [5].

Anatomical context of Aurkb

• In addition, midzone microtubule bundles became destabilized and aurora B dispersed from the equator [4].

References