Disease relevance of copB

• CopA and CopB from the thermophile Archaeoglobus fulgidus were cloned and expressed in E. coli [1].

High impact information on copB

• These and other experiments have shown that three plasmid determinants--copA/incA, copB, and copT--are involved in DNA replication control [2].
• The data suggest that the presence of the His residue in the putative transmembrane metal binding site of CopB determines a selectivity for this enzyme that is different for that observed in Cu+/Ag+-ATPases carrying a Cys-Pro-Cys sequence [3].
• Archaeoglobus fulgidus CopB is a thermophilic Cu2+-ATPase: functional role of its histidine-rich-N-terminal metal binding domain [3].
• Expression, isolation, and crystallization of the catalytic domain of CopB, a putative copper transporting ATPase from the thermoacidophilic archaeon Sulfolobus solfataricus [4].
• Based on measurements of specific activities of this fused protein under various conditions, it was concluded that expression of copB is gene dosage dependent, unregulated by plasmid-coded functions, and proportional to growth rate between 0.4 and 2.0 doublings per h [5].

Biological context of copB

• Deletions or insertions in the copB gene of plasmid R1 result in a copy mutant phenotype [6].
• Oligonucleotide primers homologous to consensus regions in the copB and repA genes, and the oriR regions from a number of IncFII-related replicons were used to amplify replication regions from pLV1402 [7].
• A second, internal transcription initiation site, the repA promoter, is usually repressed by the copB protein, and is therefore only of significance in the absence of this control factor [8].
• The CopB protein represses initiation of transcription of the repA gene, and its target site lies within a 60 base pair region containing the repA promoter [9].
• The differences in enzyme selectivity can be explained by different consensus sequences in the transmembrane cation binding domain (CopA: CPC, CopB: CPH) [1].

Associations of copB with chemical compounds

• In agreement with in vivo results the binding between CopB and its target site is moderate compared with other operons like lac and trp [10].

Other interactions of copB

• CopA encodes a small, unstable, untranslated RNA molecule that inhibits translation of the repA message whereas copB produces a protein that inhibits transcription from the repA promoter [11].

Analytical, diagnostic and therapeutic context of copB

• Southern blot analysis showed that chromosomal DNA from seven different M. catarrhalis strains hybridized with a probe comprising the majority of the copB structural gene from strain 035E [12].
• Molecular cloning and functional characterization of a copy number control gene (copB) of plasmid R1 [6].
• Additional data emphasizing the extent of conservation of the CopB protein among M. catarrhalis strains were obtained from Western immunoblot analyses with polyclonal antisera raised against CopB proteins from different M. catarrhalis strains used to probe the recombinant form of the CopB protein from strain 035E [12].

References