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Gene Review:

lrp - leucine-responsive transcriptional regulator

Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

Camacho, E.M. et al., Marshall, D.G. et al., Hecht, K. et al.

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Disease relevance of lrp

A role for the leucine-responsive regulatory protein and integration host factor in the regulation of the Salmonella plasmid virulence (spv ) locus in Salmonella typhimurium [1].

High impact information on lrp

The leucine-responsive regulatory protein (Lrp) is an activator of traJ transcription [2].
The effect of GATC-II hemimethylation on Lrp binding is strand-specific: methylation of the traJ non-coding strand permits formation of the major Lrp-DNA retardation complex, but methylation of the coding strand does not [2].
This asymmetry supports a model in which passage of the replication fork may permit Lrp-mediated activation of conjugal transfer in one daughter plasmid molecule but not in the other [2].
The upstream-activating-sequence of the pSLT traJ promoter contains two Lrp binding sites (LRP-1 and LRP-2), both necessary for transcriptional activation [2].
These are the integration host factor (IHF), which binds to DNA sequences upstream of the spvR regulatory gene, and the leucine-responsive regulatory protein (Lrp), which binds to sequences upstream of the spvABCD operon [1].

Chemical compound and disease context of lrp

D-histidine utilization in Salmonella typhimurium is controlled by the leucine-responsive regulatory protein (Lrp) [3].

Biological context of lrp

Lrp represses spvA gene expression by up to fivefold and Lrp-mediated repression is antagonized by leucine [1].
The suppressor mutations are located in the helix-turn-helix region of Lrp [3].

Associations of lrp with chemical compounds

The lrp mutations arise as suppressors of mutations in the genes encoding the histidine permease which drastically decrease the level of histidine transport activity [3].

References

A role for the leucine-responsive regulatory protein and integration host factor in the regulation of the Salmonella plasmid virulence (spv ) locus in Salmonella typhimurium. Marshall, D.G., Sheehan, B.J., Dorman, C.J. Mol. Microbiol. (1999) [Pubmed]
Regulation of traJ transcription in the Salmonella virulence plasmid by strand-specific DNA adenine hemimethylation. Camacho, E.M., Casadesús, J. Mol. Microbiol. (2005) [Pubmed]
D-histidine utilization in Salmonella typhimurium is controlled by the leucine-responsive regulatory protein (Lrp). Hecht, K., Zhang, S., Klopotowski, T., Ames, G.F. J. Bacteriol. (1996) [Pubmed]

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