Disease relevance of spvA

• In vivo analysis of the interactions of the LysR-like regulator SpvR with the operator sequences of the spvA and spvR virulence genes of Salmonella typhimurium [1].
• Outer-membrane porins from gram-negative bacteria stimulate platelet-activating-factor biosynthesis by cultured human endothelial cells [2].
• Outer membrane protein-specific bacteriophages of Salmonella typhimurium [3].

High impact information on spvA

• The spvR gene was found to possess an operator site that resembled closely the distal SpvR binding site of the spvA operator [1].
• DMS methylation protection assays showed that, in vivo, SpvR forms direct protein-DNA contacts with nucleotides clustered in two regions (+1 to -27 and -51 to -71) of the spvA regulatory region [1].
• When the nucleotide sequence of the spvR operator was altered at two nucleotides so that it corresponded more precisely to that of the distal site of the spvA operator, strong SpvR-DNA interactions were detected, with nucleotides in the region -31 to -67 being protected from DMS methylation in vivo [1].
• Outer membrane phospholipase A is dimeric in phospholipid bilayers: a cross-linking and fluorescence resonance energy transfer study [4].
• Outer membrane mutants of Salmonella typhimurium LT2 have lipopolysaccharide-dependent resistance to the bactericidal activity of anaerobic human neutrophils [5].

Chemical compound and disease context of spvA

• The interaction of the Salmonella typhimurium virulence gene regulator, SpvR, with its operator sites upstream of the spvA and spvR genes was analysed in vivo by dimethyl sulphate (DMS) footprinting and site-directed mutagenesis [1].
• Outer membrane permeability barrier disruption by polymyxin in polymyxin-susceptible and -resistant Salmonella typhimurium [6].
• Outer membrane of Salmonella typhimurium: reconstitution of sucrose-permeable membrane vesicles [7].

Anatomical context of spvA

• Outer membrane and flagella proteins induced DTH responses, whereas lipopolysaccharide failed to do so [8].

Analytical, diagnostic and therapeutic context of spvA

• Outer membrane mutants of Salmonella typhimurium deficient in one, two, or three of the 33,000-dalton (33K), 34K, and 36K outer membrane proteins (7) were studied by using thin sectioning and freeze-fracturing electron microscopy techniques [9].
• Outer membrane protein content is drastically modified after treatments [10].

References