Disease relevance of Centrifugation, Zonal

• Swarm rat chondrosarcoma proteoglycans. Purification of aggregates by zonal centrifugation of preformed cesium sulfate gradients [1].
• Rapidly sedimenting complexes (RSCs) of replicating phage K DNA, isolated by rate zonal centrifugation in sucrose gradients, contain bacterial membrane lipids and protein [2].
• Separation of DHBV from the plasma of carrier ducks by rate zonal centrifugation was examined by DNA polymerase (DNAP) activity [3].

High impact information on Centrifugation, Zonal

• Low density lipoprotein (LDL) was isolated by zonal centrifugation from the plasma of seven HTG subjects, before and 2 wk after the initiation of bezafibrate (BZ) therapy [4].
• Chicken metaphase chromosomes were partially purified by rate zonal centrifugation, and DNA was prepared from each of the fractions of the sucrose gradient [5].
• A similar value was obtained for the native protein by rate zonal centrifugation on glycerol gradients [6].
• Differential centrifugation, rate zonal centrifugation, and equilibrium sucrose gradient centrifugation of rat liver homogenates all showed that these enzyme activities did not comigrate with HMG-CoA reductase [7].
• Equilibrium as well as rate zonal centrifugation suggest that this denser [3H] retinoid-containing vesicle may represent endoplasmic reticulum [8].

Biological context of Centrifugation, Zonal

• Parenchymal cells, isolated from rat liver by a simple non-enzymic technique, were fractioned according to ploidy class by rate zonal centrifugation on sucrose density gradients [9].
• In nuclei separated by zonal centrifugation on sucrose gradients, the DFP-labeled proteins are highest in activity in the elongated spermatids at the stage in spermiogenesis at which histones are being replaced by testis-specific proteins and protamines [10].

Anatomical context of Centrifugation, Zonal

• Calcium adenosine triphosphatase (Ca-ATPase) activity in rat liver plasma membrane fractions prepared by zonal centrifugation was studied for sensitivity to carbon tetrachloride (CCl4) [11].
• LDL-receptor status in fresh monocytes, affinity of LDL towards the LDL receptor in cultured fibroblasts, zonal-centrifugation profiles, and lipoprotein composition were not significantly different between the diets [12].
• Density profile and basic protein measurements in the myelin range of particulate material from normal developing mouse brain and from neurological mutants (Jimpy; quaking; Trembler; shiverer and its mld allele) obtained by zonal centrifugation [13].
• Purification of messenger ribonucleoprotein particles from rabbit reticulocytes by zonal centrifugation in metrizamide [14].
• Two methods of preparing the soluble glyoxylate cycle enzyme are described: an elaborated method which used crude extracts of cucumber cotyledons, and another procedure which started with purified glyoxysomes from 4-day-old cotyledons and included a separation of glyoxysomal matrix enzymes by zonal centrifugation [15].

Associations of Centrifugation, Zonal with chemical compounds

• Released folates were separated from binder by Sephadex gel filtration, zonal centrifugation through sucrose gradients, or adsorption onto activated charcoal [16].
• The pure transferase has an apparent molecular weight of 100,000 as judged by zonal centrifugation and by sodium dodecyl sulfate polyacrylamide gel electrophoresis in the absence of a reducing agent [17].
• The purification procedure included zonal centrifugation through a 5 to 30% dextran t-10 gradient, followed by equilibrium centrifugation in a 5 to 50% potassium tartrate gradient [18].
• Primer-free RNA subunits obtained by heat treatment and zonal centrifugation were digested with RNase T2, and methylated oligonucleotides were chromatographed on DEAE-Sephadex in 7 M urea [19].
• Fractionation of cell-free extracts, by high-speed zonal centrifugation, of methanol-grown C. boidinii showed non-sedimentable and sedimentable proteolytic activities [20].

Gene context of Centrifugation, Zonal

• Rate zonal centrifugation suggested such complexes are more likely to involve MUC5B rather than MUC5AC mucins [21].
• H2A-H2B dimer fractions obtained from MH-134SC cells labeled with suitable precursors were fractionated by rate zonal centrifugation in sucrose gradients containing 2 M NaCl [22].
• Myelin and premyelin material denser than myelin were obtained from quaking (Qk), shiverer (Shi), and myelin-deficient (mld) mutant and control mice, using zonal centrifugation on zonal rotor [23].
• By use of rate zonal centrifugation, followed by either pulsed-field agarose gel electrophoresis or restriction endonuclease analysis, in the present study, the following observations were made [24].
• 2. After high-speed zonal centrifugation most of the protein, acid and alkaline phosphatases, alkaline pyrophosphatase, adenosine monophosphatase, beta-fructofuranosidase, alpha-mannosidase, NADPH-cytochrome c oxidoreductase and an appreciable amount of phospholipid and sterol were non-sedimentable, i.e. were at densities below 1.09 (g/cm3) [25].

Analytical, diagnostic and therapeutic context of Centrifugation, Zonal

• The product behaved as a single species when tested by (a) polyacrylamide gel electrophoresis in the presence of dodecyl sulfate, (b) zonal centrifugation, and (c) double immunodiffusion against rabbit antibody to isocitrate lyase [15].
• Antigenic substances from livers of mice infected with Tyzzer's disease were purified by means of sucrose density gradient zonal centrifugation and affinity column chromatography using antiserum and checking antigenicity with the complement fixation test [26].

References