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Gene Review

Cmas  -  cytidine monophospho-N-acetylneuraminic...

Mus musculus

Synonyms: AW208911, CMP-N-acetylneuraminic acid synthase, CMP-Neu5Ac synthase, CMP-NeuNAc synthase, CMP-sialic acid synthetase, ...
 
 
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Disease relevance of Cmas

  • A murine cDNA encoding a protein of 432 amino acids rescued the lec32 mutation and also caused polysialic acid to be expressed in the capsule of the CMP-Neu5Ac synthetase negative Escherichia coli mutant EV5 [1].
 

High impact information on Cmas

 

Biological context of Cmas

 

Anatomical context of Cmas

  • Engineering the CMP-SA metabolic pathway may be beneficial in various cell lines in which CMP-Neu5Ac production limits sialylation of glycoproteins or other glycans [6].
  • In addition, co-expression of Cmp-Sas with the recently cloned sialic acid phosphate synthase with N-acetylmannosamine feeding yields intracellular CMP-Neu5Ac levels 30 times higher than those observed in unsupplemented CHO cells [6].
  • An apparent Km of 11 microM was determined for the substrate CMP-Neu5Ac using purified hydroxylase in the presence of Triton X-100-solubilized microsomes [7].
 

Associations of Cmas with chemical compounds

  • However, examination of insect cells grown in serum-free medium revealed negligible native levels of the most common sialic acid nucleotide, CMP-N-acetylneuraminic acid (CMP-Neu5Ac) [6].
 

Analytical, diagnostic and therapeutic context of Cmas

  • Sequence analysis of the cloned murine CMP-Neu5Ac synthetase identified three clusters of basic amino acids (BC1-BC3) that might function as nuclear localization signals (NLS) [3].

References

  1. Mammalian cytidine 5'-monophosphate N-acetylneuraminic acid synthetase: a nuclear protein with evolutionarily conserved structural motifs. Münster, A.K., Eckhardt, M., Potvin, B., Mühlenhoff, M., Stanley, P., Gerardy-Schahn, R. Proc. Natl. Acad. Sci. U.S.A. (1998) [Pubmed]
  2. A mutation in human CMP-sialic acid hydroxylase occurred after the Homo-Pan divergence. Chou, H.H., Takematsu, H., Diaz, S., Iber, J., Nickerson, E., Wright, K.L., Muchmore, E.A., Nelson, D.L., Warren, S.T., Varki, A. Proc. Natl. Acad. Sci. U.S.A. (1998) [Pubmed]
  3. Nuclear localization signal of murine CMP-Neu5Ac synthetase includes residues required for both nuclear targeting and enzymatic activity. Munster, A.K., Weinhold, B., Gotza, B., Muhlenhoff, M., Frosch, M., Gerardy-Schahn, R. J. Biol. Chem. (2002) [Pubmed]
  4. Detection of CMP-N-acetylneuraminic acid hydroxylase activity in fractionated mouse liver. Shaw, L., Schauer, R. Biochem. J. (1989) [Pubmed]
  5. Ras oncogene induces beta-galactoside alpha2,6-sialyltransferase (ST6Gal I) via a RalGEF-mediated signal to its housekeeping promoter. Dalziel, M., Dall'Olio, F., Mungul, A., Piller, V., Piller, F. Eur. J. Biochem. (2004) [Pubmed]
  6. Cloning and expression of human sialic acid pathway genes to generate CMP-sialic acids in insect cells. Lawrence, S.M., Huddleston, K.A., Tomiya, N., Nguyen, N., Lee, Y.C., Vann, W.F., Coleman, T.A., Betenbaugh, M.J. Glycoconj. J. (2001) [Pubmed]
  7. Purification and characterization of CMP-N-acetylneuraminic acid hydroxylase from pig submandibular glands. Schlenzka, W., Shaw, L., Schneckenburger, P., Schauer, R. Glycobiology (1994) [Pubmed]
 
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