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Gene Review

NS2  -  NS2

Bovine respiratory syncytial virus

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Disease relevance of NS2


High impact information on NS2

  • Here we demonstrate that, in contrast to wild-type BRSVs, recombinant BRSVs (rBRSVs) lacking the NS proteins, and those lacking NS2 in particular, are strong inducers of IFN-alpha/beta in bovine nasal fibroblasts and bronchoalveolar macrophages [6].
  • In cells coinfected with NS1- and NS2-expressing rRVs, virus replication was resistant to doses of IFN which caused a 1,000-fold reduction of replication in cells infected with wild-type RV or with each of the NS-expressing rRVs alone [3].
  • Thus, RSV has the potential for two transcription programs: one in the absence of M2-1, in which only the NS1 and NS2 genes are transcribed, and one in the presence of M2-1, in which sequential transcription of the complete genome occurs [7].
  • Coexpression of the nonstructural NS1 or NS2 protein had little effect on packaging and passage except through indirect effects on RNA synthesis in the initial transfection [8].
  • Airway epithelial cells were also infected with equivalent inoculums of RSV without or with single gene deletions of NS1 or NS2 [9].

Biological context of NS2


Anatomical context of NS2

  • The antipeptide antibodies recognized the NS2 protein in the sediment of RSV-infected HEp-2 cells lysed with RIPA buffer at 48 h p.i. The results agree with the reported interaction of RSV with cytoskeletal intermediate filaments [11].

Other interactions of NS2

  • In addition, we report the successful recovery of a BRSV mutant lacking the complete NS2 gene, which encodes a nonstructural protein of unknown function [1].

Analytical, diagnostic and therapeutic context of NS2


  1. Generation of bovine respiratory syncytial virus (BRSV) from cDNA: BRSV NS2 is not essential for virus replication in tissue culture, and the human RSV leader region acts as a functional BRSV genome promoter. Buchholz, U.J., Finke, S., Conzelmann, K.K. J. Virol. (1999) [Pubmed]
  2. Rescue of synthetic minireplicons establishes the absence of the NS1 and NS2 genes from avian pneumovirus. Randhawa, J.S., Marriott, A.C., Pringle, C.R., Easton, A.J. J. Virol. (1997) [Pubmed]
  3. Bovine respiratory syncytial virus nonstructural proteins NS1 and NS2 cooperatively antagonize alpha/beta interferon-induced antiviral response. Schlender, J., Bossert, B., Buchholz, U., Conzelmann, K.K. J. Virol. (2000) [Pubmed]
  4. mRNA sequence of three respiratory syncytial virus genes encoding two nonstructural proteins and a 22K structural protein. Elango, N., Satake, M., Venkatesan, S. J. Virol. (1985) [Pubmed]
  5. Suppression of the induction of alpha, beta, and lambda interferons by the NS1 and NS2 proteins of human respiratory syncytial virus in human epithelial cells and macrophages [corrected]. Spann, K.M., Tran, K.C., Chi, B., Rabin, R.L., Collins, P.L. J. Virol. (2004) [Pubmed]
  6. Role of alpha/beta interferons in the attenuation and immunogenicity of recombinant bovine respiratory syncytial viruses lacking NS proteins. Valarcher, J.F., Furze, J., Wyld, S., Cook, R., Conzelmann, K.K., Taylor, G. J. Virol. (2003) [Pubmed]
  7. Role of the M2-1 transcription antitermination protein of respiratory syncytial virus in sequential transcription. Fearns, R., Collins, P.L. J. Virol. (1999) [Pubmed]
  8. Identification of the respiratory syncytial virus proteins required for formation and passage of helper-dependent infectious particles. Teng, M.N., Collins, P.L. J. Virol. (1998) [Pubmed]
  9. Respiratory syncytial virus nonstructural protein 2 specifically inhibits type I interferon signal transduction. Ramaswamy, M., Shi, L., Varga, S.M., Barik, S., Behlke, M.A., Look, D.C. Virology (2006) [Pubmed]
  10. Nucleotide sequence analysis of the non-structural NS1 (1C) and NS2 (1B) protein genes of bovine respiratory syncytial virus. Pastey, M.K., Samal, S.K. J. Gen. Virol. (1995) [Pubmed]
  11. Nonstructural protein 2 (NS2) of respiratory syncytial virus (RSV) detected by an antipeptide serum. Weber, E., Humbert, B., Streckert, H.J., Werchau, H. Respiration; international review of thoracic diseases. (1995) [Pubmed]
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