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MeSH Review

Radioimmunoprecipitation Assay

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Disease relevance of Radioimmunoprecipitation Assay


High impact information on Radioimmunoprecipitation Assay

  • Increased surface expression of alpha receptor subunit by TGF-beta in SSc fibroblasts correlated with increased new PDGF alpha receptor synthesis as demonstrated by radioimmunoprecipitation analysis of metabolically labeled cells and with increased steady-state levels of corresponding mRNAs [6].
  • A double-antibody radioimmunoprecipitation assay was used to measure the rate of incorporation of radioactive precursors into carcinoembryonic antigen (CEA) newly synthesized by cultured human colon carcinoma (LoVo) cells [7].
  • Antibodies to mite (Der f 1) and cat (Fel d 1) allergens measured by isotype (IgG and IgG4) specific radioimmunoprecipitation assays were compared with sensitisation and allergen concentrations in house dust [8].
  • Greater than 80% of the cadherin in the cell is cleared from Triton X-100 lysates of MvLu cells after immunoprecipitation with antibodies to PTPmu; however, the complex is dissociated when lysates are prepared in more stringent, SDS-containing RIPA buffer [9].
  • Four of five ELISA-reactive serum samples were negative by HTLV-I immunoblot assay (IB); 1 reactive and 1 borderline reactive serum were indeterminate on IB (p19 reactivity), but negative by radioimmunoprecipitation assay (RIPA) [10].

Chemical compound and disease context of Radioimmunoprecipitation Assay


Biological context of Radioimmunoprecipitation Assay

  • We report three members of a family who had reduced levels of plasma von Willebrand factor (vWF) and increased ristocetin-induced platelet aggregation (RIPA) (aggregation of platelet-rich plasma with ristocetin at a concentration of 0.45 mg/mL), as previously reported in type IIB and pseudo-von Willebrand's disease (vWD) [16].
  • A radioimmunoprecipitation assay was used to study antibody responses to parainfluenza virus 3 glycoproteins in human sera [17].
  • Cross-reactions against the env glycoproteins were observed by WB in 10% (7 of 70) and by RIPA in 40% (28 of 70) of the HIV-1 antibody-positive serum samples and by WB in 29% (12 of 42) and by RIPA in 48% (20 of 42) of the HIV-2 antibody-positive serum samples [18].
  • Most immunobiochemical studies, whose objective is to study the functional relevance of tyrosine phosphorylation are, however, performed using the supernatants of cells that are lysed in non-ionic detergent-containing buffers (RIPA lysis buffers) [19].
  • Abnormal hemostatic findings included a prolonged bleeding time (BT), decreased levels of factor VIII coagulant activity (VIIIC), von Willebrand factor antigen (vWF:Ag), ristocetin cofactor (RCof), and an increased RIPA [20].

Anatomical context of Radioimmunoprecipitation Assay

  • In radioimmunoprecipitation assays two of the Fabs, MV12 and MT14, precipitated an approximately equal 80-kDa protein band corresponding to the hemagglutinin (H) protein from MV-infected Vero cell cultures, while two other Fabs, MT64 and GL29, precipitated an approximately equal 60-kDa protein corresponding the nucleocapsid (N) protein [21].
  • The strongest relation with clinico-pathological data, such as lesion size, lymph node involvement, and prognosis, was found for E7 synthetic-peptide ELISA, whereas E6 and E7 RIPA did not reach significance [22].
  • Primary immune response to these antigens was studied at the humoral level (by the Farr assay) and at the cellular level (by the rosette and the plaque assays using lysozyme coupled to sheep or pigeon erythrocytes) [23].
  • Furthermore, HIV tat was transfected into HeLa cells (containing 10-20 copies per cell of HPV18), and HPV18 E7 protein expression was evaluated by a radioimmunoprecipitation assay using polyclonal antibodies against the E7 protein [24].
  • Thrombocyte count was unchanged after DDAVP infusion and RIPA was always diminished [25].

Associations of Radioimmunoprecipitation Assay with chemical compounds

  • In this method, polyethylene glycol precipitation of formed 3H-DNA/antiDNA complexes is used instead of the ammonium sulfate precipitation used in the Farr assay [26].
  • Proteolytic processing of caspases-3 and -7 and the caspase substrates poly(ADP-ribose) polymerase, GDP dissociation inhibitor, and PKCdelta was observed when PBLs were activated in vitro, and lysates were prepared using RIPA buffer which contains 1% Nonidet P-40, 0.5% deoxycholate, and 0.1% SDS [27].
  • The Farr assay and sucrose gradient ultracentrifugation were used to study the importance of kinetic factors on the size of antibody/DNA immune complexes prepared from SLE sera and dsDNA [28].
  • Radioimmunoprecipitation analysis of the 42-kDa glycoprotein, which was previously shown to represent the ISAV hemagglutinin, indicated that this protein exists at least as dimers [29].
  • As A23187, but not phorbol myristate acetate (0.1 microM) inhibited RIPA, an increase in intracellular calcium ions rather than direct stimulation of protein kinase C appears to mediate agonist-induced inhibition [30].

Gene context of Radioimmunoprecipitation Assay


Analytical, diagnostic and therapeutic context of Radioimmunoprecipitation Assay


  1. Serologic identification and characterization of a macaque T-lymphotropic retrovirus closely related to HTLV-III. Kanki, P.J., McLane, M.F., King, N.W., Letvin, N.L., Hunt, R.D., Sehgal, P., Daniel, M.D., Desrosiers, R.C., Essex, M. Science (1985) [Pubmed]
  2. Prevalence of antibodies to lymphadenopathy-associated retrovirus in African patients with AIDS. Brun-Vézinet, F., Rouzioux, C., Montagnier, L., Chamaret, S., Gruest, J., Barré-Sinoussi, F., Geroldi, D., Chermann, J.C., McCormick, J., Mitchell, S. Science (1984) [Pubmed]
  3. Molecular characterization of receptor binding proteins and immunogens of virulent Treponema pallidum. Baseman, J.B., Hayes, E.C. J. Exp. Med. (1980) [Pubmed]
  4. Von Willebrand's disease with spontaneous platelet aggregation induced by an abnormal plasma von Willebrand factor. Grainick, H.R., Williams, S.B., McKeown, L.P., Rick, M.E., Maisonneuve, P., Jenneau, C., Sultan, Y. J. Clin. Invest. (1985) [Pubmed]
  5. Avidity of anti-DNA antibodies in serum and IgG glomerular eluates from patients with systemic lupus erythematosus. Association of high avidity antinative DNA antibody with glomerulonephritis. Winfield, J.B., Faiferman, I., Koffler, D. J. Clin. Invest. (1977) [Pubmed]
  6. Selective upregulation of platelet-derived growth factor alpha receptors by transforming growth factor beta in scleroderma fibroblasts. Yamakage, A., Kikuchi, K., Smith, E.A., LeRoy, E.C., Trojanowska, M. J. Exp. Med. (1992) [Pubmed]
  7. A method to measure carcinoembryonic antigen neosynthesis by cultured colon carcinoma cells. Yang, L.Y., Drewinko, B. J. Natl. Cancer Inst. (1980) [Pubmed]
  8. Sensitisation, asthma, and a modified Th2 response in children exposed to cat allergen: a population-based cross-sectional study. Platts-Mills, T., Vaughan, J., Squillace, S., Woodfolk, J., Sporik, R. Lancet (2001) [Pubmed]
  9. Receptor protein tyrosine phosphatase PTPmu associates with cadherins and catenins in vivo. Brady-Kalnay, S.M., Rimm, D.L., Tonks, N.K. J. Cell Biol. (1995) [Pubmed]
  10. Absence of human T-cell lymphotropic virus type I coinfection in human immunodeficiency virus-infected hemophilic men. Lairmore, M.D., Jason, J.M., Hartley, T.M., Khabbaz, R.F., De, B., Evatt, B.L. Blood (1989) [Pubmed]
  11. Specificity of human antibodies to oncovirus glycoproteins: recognition of antigen by natural antibodies directed against carbohydrate structures. Snyder, H.W., Fleissner, E. Proc. Natl. Acad. Sci. U.S.A. (1980) [Pubmed]
  12. Development and evaluation of immunoassay for detection of antibodies to the feline T-lymphotropic lentivirus (feline immunodeficiency virus). O'Connor, T.P., Tanguay, S., Steinman, R., Smith, R., Barr, M.C., Yamamoto, J.K., Pedersen, N.C., Andersen, P.R., Tonelli, Q.J. J. Clin. Microbiol. (1989) [Pubmed]
  13. A radioactive antigen-binding assay for the measurement of antibody to Haemophilus influenzae type b capsular polysaccharide. Kuo, J.S., Monji, N., Schwalbe, R.S., McCoy, D.W. J. Immunol. Methods (1981) [Pubmed]
  14. Adjuvant effect of bacterial LPS and/or alum precipitation in responses to polysaccharide and protein antigens. Seppälä, I.J., Mäkelä, O. Immunology (1984) [Pubmed]
  15. A comparison of serologic reactivity among SLE patients with or without anti-Ro (SS-A) antibodies. Bell, D.A., Komar, R., Chodirker, W.B., Block, J., Cairns, E. J. Rheumatol. (1984) [Pubmed]
  16. A new von Willebrand variant (type I, New York): increased ristocetin-induced platelet aggregation and plasma von Willebrand factor containing the full range of multimers. Weiss, H.J., Sussman, I.I. Blood (1986) [Pubmed]
  17. Acquisition of serum antibodies to specific viral glycoproteins of parainfluenza virus 3 in children. Kasel, J.A., Frank, A.L., Keitel, W.A., Taber, L.H., Glezen, W.P. J. Virol. (1984) [Pubmed]
  18. Envelope cross-reactivity between human immunodeficiency virus types 1 and 2 detected by different serological methods: correlation between cross-neutralization and reactivity against the main neutralizing site. Böttiger, B., Karlsson, A., Andreasson, P.A., Nauclér, A., Costa, C.M., Norrby, E., Biberfeld, G. J. Virol. (1990) [Pubmed]
  19. Immunoblotting and sequential lysis protocols for the analysis of tyrosine phosphorylation-dependent signaling. Gilbert, C., Rollet-Labelle, E., Caon, A.C., Naccache, P.H. J. Immunol. Methods (2002) [Pubmed]
  20. A probable double heterozygous type II von Willebrand's disease with increased ristocetin induced platelet aggregation. Kinoshita, S., Yoshioka, K., Kasahara, M., Takamiya, O. Am. J. Hematol. (1992) [Pubmed]
  21. Neutralizing human Fab fragments against measles virus recovered by phage display. de Carvalho Nicacio, C., Williamson, R.A., Parren, P.W., Lundkvist, A., Burton, D.R., Björling, E. J. Virol. (2002) [Pubmed]
  22. Relation between HPV-16 serology and clinico-pathological data in cervical carcinoma patients: prognostic value of anti-E6 and/or anti-E7 antibodies. Baay, M.F., Duk, J.M., Groenier, K.H., Burger, M.P., de Bruijn, H.W., Hollema, H., Stolz, E., Herbrink, P. Cancer Immunol. Immunother. (1997) [Pubmed]
  23. Studies on B- and T-cell receptors for lysozyme. Charreire, J., Faure, A., Bach, J.F. Immunology (1975) [Pubmed]
  24. Human immunodeficiency virus type 1 tat gene enhances human papillomavirus early gene expression. Tornesello, M.L., Buonaguro, F.M., Beth-Giraldo, E., Giraldo, G. Intervirology (1993) [Pubmed]
  25. Diagnosis and differentiation of von Willebrand's disease type II. Lenk, H., Nilsson, I.M., Holmberg, L., Weissbach, G. Folia Haematol. Int. Mag. Klin. Morphol. Blutforsch. (1988) [Pubmed]
  26. Specificity in systemic lupus erythematosus of antibodies to double-stranded DNA measured with the polyethylene glycol precipitation assay. Smeenk, R., van der Lelij, G., Swaak, T., Groenwold, J., Aarden, L. Arthritis Rheum. (1982) [Pubmed]
  27. Granzyme release and caspase activation in activated human T-lymphocytes. Zapata, J.M., Takahashi, R., Salvesen, G.S., Reed, J.C. J. Biol. Chem. (1998) [Pubmed]
  28. Stability of DNA/anti-DNA complexes. III. Kinetic control of immune complex size. Taylor, R.P., Waller, S.J., Haden, C., Addis, D.J. J. Immunol. (1980) [Pubmed]
  29. Identification and characterization of viral structural proteins of infectious salmon anemia virus. Falk, K., Aspehaug, V., Vlasak, R., Endresen, C. J. Virol. (2004) [Pubmed]
  30. The effect of agonists and antagonists of platelet aggregation on von Willebrand factor-mediated platelet agglutination. McPherson, J., Zucker, M.B., Mauss, E.A., Brownlea, S. Thromb. Haemost. (1991) [Pubmed]
  31. Lack of immortalizing activity of a human papillomavirus type 16 variant DNA with a mutation in the E2 gene isolated from normal human cervical keratinocytes. Storey, A., Greenfield, I., Banks, L., Pim, D., Crook, T., Crawford, L., Stanley, M. Oncogene (1992) [Pubmed]
  32. Analysis of antibody reactivity against cysteine sulfinic acid decarboxylase, a pyridoxal phosphate-dependent enzyme, in endocrine autoimmune disease. Sköldberg, F., Rorsman, F., Perheentupa, J., Landin-Olsson, M., Husebye, E.S., Gustafsson, J., Kämpe, O. J. Clin. Endocrinol. Metab. (2004) [Pubmed]
  33. Increased basigin in bleomycin-induced lung injury. Betsuyaku, T., Kadomatsu, K., Griffin, G.L., Muramatsu, T., Senior, R.M. Am. J. Respir. Cell Mol. Biol. (2003) [Pubmed]
  34. Serologic and genetic markers of celiac disease: a sequential study in the screening of first degree relatives. Bonamico, M., Ferri, M., Mariani, P., Nenna, R., Thanasi, E., Luparia, R.P., Picarelli, A., Magliocca, F.M., Mora, B., Bardella, M.T., Verrienti, A., Fiore, B., Uccini, S., Megiorni, F., Mazzilli, M.C., Tiberti, C. J. Pediatr. Gastroenterol. Nutr. (2006) [Pubmed]
  35. Viral proteins VP2, VP6, and NSP2 are strongly precipitated by serum and fecal antibodies from children with rotavirus symptomatic infection. Colomina, J., Gil, M.T., Codoñer, P., Buesa, J. J. Med. Virol. (1998) [Pubmed]
  36. Production and characterization of species-specific monoclonal antibodies against Leishmania donovani for immunodiagnosis. Jaffe, C.L., Bennett, E., Grimaldi, G., McMahon-Pratt, D. J. Immunol. (1984) [Pubmed]
  37. Evidence of anaphylaxy after alteplase infusion. Rudolf, J., Grond, M., Prince, W.S., Schmülling, S., Heiss, W.D. Stroke (1999) [Pubmed]
  38. Contribution of polymerase chain reaction and radioimmunoprecipitation assay in the confirmation of human T-lymphotropic virus infection in French blood donors. Retrovirus Study Group of the French Society of Blood Transfusion. Defer, C., Coste, J., Descamps, F., Voisin, S., Lemaire, J.M., Maniez, M., Couroucé, A.M. Transfusion (1995) [Pubmed]
  39. Radioimmunoassay to detect antitransglutaminase autoantibodies is the most sensitive and specific screening method for celiac disease. Bonamico, M., Tiberti, C., Picarelli, A., Mariani, P., Rossi, D., Cipolletta, E., Greco, M., Tola, M.D., Sabbatella, L., Carabba, B., Magliocca, F.M., Strisciuglio, P., Di Mario, U. Am. J. Gastroenterol. (2001) [Pubmed]
  40. Human papillomavirus-related serological markers of invasive cervical carcinoma in Brazil. Sun, Y., Eluf-Neto, J., Bosch, F.X., Muñoz, N., Booth, M., Walboomers, J.M., Shah, K.V., Viscidi, R.P. Cancer Epidemiol. Biomarkers Prev. (1994) [Pubmed]
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