Disease relevance of Span

• Vascular endothelial cells in both adrenal tumors and cutaneous Kaposi's sarcomas lesions are MAb 4A11 reactive [1].

High impact information on Span

• MAb 4A11 detects endothelial cells in diseased tissues such as rheumatoid and osteoarthritic synovium and psoriatic skin [1].
• MAb 4A11 reacts with most vascular endothelial cells in lymphoid tissue but with few (< 10%) endothelial cells in thymus, spleen, liver, lung, adrenal gland, placenta, testes, and skin [1].
• MAb 4A11 does not react with several myeloid or lymphoid cell lines or with peripheral blood cells [1].
• In contrast to emulsions containing Span, long chain polyoxyethylene of Brij showed decreased transfection activity [2].
• Emulsions that were stable even after heating or freezing resulted when equal volumes of hexadecane and a 70:30 blend of Tween 80/Span 80(T80/S80) were mixed and then diluted with normal saline solution to the desired hexadecane concentration [3].

Biological context of Span

• By screening a mouse testis cDNA library with MAb 4A11, one positive clone (964bp) was obtained [4].

Analytical, diagnostic and therapeutic context of Span

• Western blotting showed a single band of 67 kDa as the corresponding antigen protein of MAb 4A11 [4].
• Combination of influenza vaccine with the Tween/Span stabilized emulsions has resulted in significantly higher antibody titers to vaccine in an extensive series of naive animal models [5].
• Using thin layer chromatography, MAb 4A11 reacts with the H-5-2 [Fuc alpha 2Gal beta 4GlcNAc beta 3Gal beta 4Glc beta 1Cer] and Lewis(y)-6 [Fuc alpha 2Gal beta 4(Fuc alpha 3)GlcNAc beta 3Gal beta 4-Glc beta 1Cer] blood group glycolipids [1].

References