Disease relevance of WAPAL

• Thus, mammalian WAPL such as hWAPL and mWAPL may be involved in spermatogenesis and be target genes mediating the reproductive toxicity induced by TCDD [1].

High impact information on WAPAL

• Wapl depletion also increases the residence time of cohesin on chromatin in interphase [2].
• Wapl is associated with cohesin throughout the cell cycle, and its depletion blocks cohesin dissociation from chromosomes during the early stages of mitosis and prevents the resolution of sister chromatids until anaphase, which occurs after a delay [2].
• Wapl takes cohesin off chromosome arms [3].
• A dioxin sensitive gene, mammalian WAPL, is implicated in spermatogenesis [1].
• We have isolated a mouse homolog of the hWAPL gene, termed mouse WAPL (mWAPL), as a target of TCDD by cDNA representational difference analysis from mouse embryonic stem cells [1].

Biological context of WAPAL

• The human papillomavirus E6 and E7 inducible oncogene, hWAPL, exhibits potential as a therapeutic target [4].
• Flow cytometric analysis suggested that the alteration of hWAPL mRNA levels is independent of cell cycle profile [5].
• In recent articles published in Cell and Current Biology, the characterization of Wapl, a newly identified cohesin-interacting protein, suggests that a dynamic interaction between the cohesin complex and chromatin is important for normal regulation of sister chromatid cohesion [6].

Anatomical context of WAPAL

• Thus, some effects from 3-MC exposure on uterus may be mediated by the unscheduled overexpression of hWAPL [5].
• Here, we demonstrate that hWAPL transcription is induced by 3-methylcholanthrene (3-MC) in the cervical carcinoma-derived cell line SiHa. hWAPL transcription was analyzed with evaluation of the mRNA and heterogeneous nuclear RNA (hnRNA) levels by quantitative real time PCR analysis [5].

Associations of WAPAL with chemical compounds

• We also found that DMSO and some components of FBS affect hWAPL transcription [5].

References