High impact information on Atxn1

• Sca-1 expression identifies stem cells in the proximal region of prostatic ducts with high capacity to reconstitute prostatic tissue [1].
• METHODS: Mesenchymal cells were isolated from perichondrium/periosteum, bone marrow or fat of adult rats and found to be positive for the stem-cell-related antigens Sca-1, c-Kit, CD10, CD13 and CD90 by reverse transcription polymerase chain reaction (RT-PCR) [2].
• Oxidative stimuli affect polyglutamine aggregation and cell death in human mutant ataxin-1-expressing cells [3].
• Ethanol significantly reduced the numbers of cells expressing the stem cell markers CD117, CD133, Sca-1 and ABCG2, without decreasing nestin expression [4].
• Most clones were the spinocerebellar ataxia type-1 (SCA1) gene encoding ataxin-1, the abnormality of which causes neurodegeneration and loss of cerebellar Purkinje cells [5].

Biological context of Atxn1

• Oxidative damage of mutant ataxin-1 protein increased its recruitment in nuclear aggregates and increased cell death [3].

Analytical, diagnostic and therapeutic context of Atxn1

• Sorted BM cells showed positive for Sca-1 (Ataxin-1) by immunofluorescence staining [6].
• By using direct immunocytochemistry of BrU incorporated to RNA in the nuclei, we evaluated the effect of mutant huntingtin and ataxin-1 on general transcription in primary cortical and cerebellar neurons [7].

References