Disease relevance of SGEF

• In addition, we report that SopB stimulates cellular responses by activating SH3-containing guanine nucleotide exchange factor (SGEF), an exchange factor for RhoG, which we found plays a central role in the actin cytoskeleton remodeling stimulated by Salmonella [1].
• In searching for androgen-responsive genes in human prostate cancer cells, we have isolated two cDNAs that encode alternate forms of a novel Src homology 3 domain-containing guanine nucleotide exchange factor (SGEF) [2].

High impact information on SGEF

• Full-length SGEF activated RhoG, but not Rac, when expressed in fibroblasts [3].
• Scanning electron microscopy of fibroblasts demonstrated that SGEF induced dorsal ruffles that were morphologically similar to those generated by constitutively active RhoG, but not constitutively active Rac1 [3].
• Transient expression of SGEF stimulated fibroblast uptake of 10-kDa dextran, a marker of macropinocytosis [3].
• SGEF (SH3-containing Guanine Nucleotide Exchange Factor) is a RhoGEF of unknown function [3].
• SGEF required its proline-rich amino-terminus to generate dorsal, but not lateral, membrane ruffles and a functional SH3 domain to colocalize with filamentous actin at sites of membrane protrusion [3].

Biological context of SGEF

• The SGEF gene is located on chromosome 3q25.2 and consists of at least 15 exons [2].
• Up-regulation of CSGEF was sensitive to actinomycin D but did not require new protein synthesis [2].

Anatomical context of SGEF

• We found the SGEF protein to be expressed in many established cell lines and highly expressed in human liver tissue [3].

Associations of SGEF with chemical compounds

• The SGEF mRNA is widely expressed in human tissues, and the predicted 871-amino acid SGEF protein contains Dbl homology and pleckstrin homology domains as well as an N-terminal proline-rich domain, a C-terminal Src homology 3 domain, and two nuclear localization signals [2].

Other interactions of SGEF

• SGEF, a RhoG guanine nucleotide exchange factor that stimulates macropinocytosis [3].
• One included Neogenin, WASF1 and SGEF genes, which may have a role in differentiation or development [4].

References