Disease relevance of GNL1

• Coronaviridae and SARS-associated coronavirus strain HSR1 [1].

High impact information on GNL1

• Comparative studies of the human and pig sequences of the class I-containing segments indeed revealed the presence within the human HSR1-ZNF segment of relics of a human class I fossil gene which appeared to be orthologous to the 5' moiety of the SLA-11 pseudogene [2].
• However, detailed investigation of the biology of 11 beta HSD-1 in liver, its function, regulation, and indeed even reaction direction, has been hampered by the lack of clonal hepatic cell lines that express 11 beta HSR-1 [3].
• Sulfate-reducing bacteria (SRB) HSR1, HSR4, and HSR14 isolated from the salt pans of Goa grew best at 90-100/1000 salinity on substrates like formate, acetate, lactate, butyrate, ethanol and benzoate [4].
• Moreover, assay of mitogenic activity shows that GNL-1 is a strong mitogen, but GNL-2 is lack of the activity [5].
• Two lectins, GNL-1 and 2, were isolated from extracts of Great Northern bean powder through fractionation with ammonium sulfate, ion-exchange chromatographies on CM- and DEAE-celluloses, and gel filtration chromatography on Sephacryl S-200 HR [5].

Anatomical context of GNL1

• The Ki-1/120, devoid of the Ki-1/57 molecule, was immunoprecipitated from cell lysates of Hodgkin-analogous cell lines L428 or L540, which had been loaded with the Ki-1 or the Ki-1-analogous antibodies Ber-H2, HSR-1, -2 and -3 (method 1) [6].

Analytical, diagnostic and therapeutic context of GNL1

• For this purpose, we have evaluated the inactivation of SARS-coronavirus (CoV) in platelet concentrates using an approved pathogen inactivation device, the INTERCEPT Blood System. Apheresis platelet concentrates (APCs) were inoculated with approximately 10(6) pfu mL(-1) of either Urbani or HSR1 isolates of SARS-CoV [7].
• The lectins (GNL-1 and 2) have molecular masses of 175 and 145 kDa on gel filtration, respectively [5].
• They yield three bands having the respective same molecular masses on SDS-PAGE (GNL-1; alpha-subunit of 34.5 kDa, beta of 37.0, and gamma of 39.0: GNL-2; alpha' of 34.5 kDa, beta' of 37.0, and gamma' of 39.0) [5].

References