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Gene Review:

PTRF - polymerase I and transcript release factor

Homo sapiens

Synonyms: CAVIN, CAVIN1, CGL4, Cavin-1, FKSG13, ...

Aboulaich, N. et al., Aboulaich, N. et al., Hasegawa, T. et al., Jansa, P. et al.

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High impact information on PTRF

Substitution of 3'-terminal uridylates by guanine residues abolishes PTRF binding and impairs release activity [1].
PTRF (polymerase I and transcript-release factor) is tissue-specific and interacts with the BFCOL1 (binding factor of a type-I collagen promoter) zinc-finger transcription factor which binds to the two mouse type-I collagen gene promoters [2].
One cDNA contained a potential open reading frame for a polypeptide of 392 amino acids and was identical to PTRF (polymerase I and transcript-release factor), which is involved in transcription termination of the RNA polymerase I reaction [2].
Polymerase I and transcript release factor (PTRF) is in human adipocytes mainly localized at the plasma membrane [3].
In the plasma membrane PTRF was specifically bound to a triacylglycerol-metabolizing subclass of caveolae containing hormone-sensitive lipase (HSL) [3].

Biological context of PTRF

The phosphorylation sites were localized to Ser-36, Ser-40, Ser-365 and Ser-366 in PTRF [4].
Vectorial proteomics reveal targeting, phosphorylation and specific fragmentation of polymerase I and transcript release factor (PTRF) at the surface of caveolae in human adipocytes [4].
The findings indicate also a novel extranuclear function for PTRF in the control of lipolysis [3].

Anatomical context of PTRF

PTRF was present at the surface of caveolae in the intact form and in five different truncated forms [4].
Caveolae of human adipocytes are proposed to function in targeting, relocation and proteolytic control of PTRF and other PEST-domain-containing signalling proteins [4].
In response to insulin PTRF was translocated to the cytosol in parallel with HSL [3].

Other interactions of PTRF

In particular, PTRF (polymerase I and transcript release factor) was found as a major caveolae-associated protein and its co-localization with caveolin was confirmed by immunofluorescence confocal microscopy [4].
Association and insulin regulated translocation of hormone-sensitive lipase with PTRF [3].

Analytical, diagnostic and therapeutic context of PTRF

Here we describe the molecular cloning of a cDNA corresponding to murine PTRF [1].

References

Cloning and functional characterization of PTRF, a novel protein which induces dissociation of paused ternary transcription complexes. Jansa, P., Mason, S.W., Hoffmann-Rohrer, U., Grummt, I. EMBO J. (1998) [Pubmed]
PTRF (polymerase I and transcript-release factor) is tissue-specific and interacts with the BFCOL1 (binding factor of a type-I collagen promoter) zinc-finger transcription factor which binds to the two mouse type-I collagen gene promoters. Hasegawa, T., Takeuchi, A., Miyaishi, O., Xiao, H., Mao, J., Isobe, K. Biochem. J. (2000) [Pubmed]
Association and insulin regulated translocation of hormone-sensitive lipase with PTRF. Aboulaich, N., Ortegren, U., Vener, A.V., Str??lfors, P. Biochem. Biophys. Res. Commun. (2006) [Pubmed]
Vectorial proteomics reveal targeting, phosphorylation and specific fragmentation of polymerase I and transcript release factor (PTRF) at the surface of caveolae in human adipocytes. Aboulaich, N., Vainonen, J.P., Strålfors, P., Vener, A.V. Biochem. J. (2004) [Pubmed]

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