High impact information on Gpx2

• Semi-quantitative PCR performed previously demonstrated RNA expression of Gpx1 and Gpx2 in proximal, transverse and distal colon [1].
• In situ hybridisation and immunohistochemistry were consistent with reports of microanatomical location of Gpx2 in the lumenal epithelium [1].
• Novel sites of Gpx2 expression were also observed in lymphatic tissue [1].
• These results support the potential for Gpx1 and Gpx2 redundancy in lymphatic tissue, but not in epithelial cells of the colon crypt or in the lamina propria, sub-mucosa, muscularis or serosa [1].
• We have determined the level of GSHPx-GI mRNA expression in the rat gastrointestinal tract from esophagus to colon [2].

Anatomical context of Gpx2

• The expression of an intestinal form of glutathione peroxidase (GSHPx-GI) in rat intestinal epithelium [2].
• This suggests that GSHPx-GI is the major cellular tetrameric GSHPx expressed in intestinal epithelium, and the expression of GSHPx-GI in the GI tract is not likely regulated differentially through maturation of epithelial cells [2].
• We have also determined the levels of GSHPx-GI mRNA expression and several antioxidant enzyme activities along the villus-to-crypt axis in the rat small intestine by cell fractionation [2].
• Although GSHPx-GI mRNA is readily detectable throughout the GI tract, the highest level is detected in the ileum and cecum [2].

Other interactions of Gpx2

• This study reports evidence of both common sites of Gpx1 and Gpx2 expression in rat colon and sites that are exclusive to each isoform [1].

References