High impact information on Hnrpa1

• Identification of a nucleic acid helix-destabilizing protein from rat liver as lactate dehydrogenase-5 [1].
• Further support for this latter idea derives from chemical-modification studies which demonstrate that tyrosine-238, which is located near the coenzyme binding site of LDH, seems to be essential for the ability of HDP to bind ss DNA [1].
• Immunoreactive helix-destabilizing protein localized in transcriptionally active regions of Drosophila polytene chromosomes [2].
• A cDNA library of newborn rat brain poly(A+) RNA in lambda gt 11 was screened with a synthetic oligonucleotide probe corresponding to a five amino acid sequence in the N-terminal region of the calf helix-destabilizing protein, UP1 [3].
• The levels of mRNA encoding hnRNP core protein A1 have been compared in quiescent and proliferating Rat-1 embryonic fibroblasts [4].

Biological context of Hnrpa1

• There was a gradual decrease in HDP mRNA content beyond day 18 of gestation, as lung maturation increased [5].

Anatomical context of Hnrpa1

• Western blot analysis of the protein extract from isolated Chironomus tentans salivary gland nuclei with anti-HDP (rat) sera showed cross-reactive protein bands with molecular masses of about 33,000, 42,000 and 65,000 daltons [6].
• Similarly, a lower content of HDP mRNA was observed in maternal heart and brain tissues than in fetal heart and brain tissues from day 18 of gestation [5].
• The five kinds of prosthetic materials: powders of cobalt alloy, stainless steel, alumina ceramic, HDP plastic and bone cement, were injected to the knee joints of 16 rats for each material [7].

Associations of Hnrpa1 with chemical compounds

• Maternal dexamethasone treatment that promotes lung maturation did not have an impact on HDP expression [5].

Analytical, diagnostic and therapeutic context of Hnrpa1

• HPLC tryptic peptide maps were also identical for both the rat liver HDP and LDH proteins [1].
• Indirect localization by immunofluorescence showed immunoreactive HDP to be preferentially concentrated on transcriptionally active Balbiani rings 1 and 2 [6].
• In correlative experiments, microinjection of the HDP itself showed stimulated transcription of all RNAs [6].

References