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Gene Review:

GUCA2B - guanylate cyclase activator 2B (uroguanylin)

Homo sapiens

Synonyms: GCAP-II, Guanylate cyclase activator 2B, UGN

Hess, R. et al., Hidaka, Y. et al., Mägert, H.J. et al., Hill, O. et al.

Mägert, Reinecke, David, Raab, Adermann, Zucht, Hill, Hess, Forssmann, Hidaka, Shimono, Ohno, Okumura, Adermann, Forssmann, Shimonishi,

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Disease relevance of GUCA2B

Five mutant proteins, in which amino acid residues were sequentially deleted from the N terminus, and three mutant proteins of pro-GCAP-II, in which N-terminal 6, 11, or 17 amino acid residues were deleted, were overproduced using Escherichia coli or human kidney 293T cells, respectively [1].

High impact information on GUCA2B

These data indicate that the propeptide has two roles in proper folding: the disulfide-coupled folding of the mature region and the dimerization of pro-GCAP-II [1].
The 16 C-terminal amino acids are identical to uroguanylin from human urine. native and synthetic GCAP-II activate GC-C, the specific guanylate cyclase receptor, of cultured human colon carcinoma (T84) cells [2].
GCAP-II stimulates chloride secretion in isolated human intestinal mucosa mediated by intracellular cGMP increase [2].
In the present study we describe the purification and amino acid sequence of a new guanylate cyclase C activating peptide (GCAP-II) [2].
GCAP-II specific antibodies were used to localize the peptide by immunohistochemistry in entero-endocrine cells of the colonic mucosa [2].

Biological context of GUCA2B

Here, we report the three exon/two intron structure of the human uroguanylin/GCAP-II gene and its localization on chromosome 1 p35-34, as determined by radiation hybrid mapping [3].
Guanylin/GCAP-I and uroguanylin/GCAP-II are two structurally related peptides which play an important role in the regulation of water/electrolyte balance within the gut [3].

Anatomical context of GUCA2B

Northern hybridization revealed that the expression of the uroguanylin/GCAP-II gene is highest in the intestinal mucosa, especially in the ileum and colon [3].

Associations of GUCA2B with chemical compounds

GCAP-II: isolation and characterization of the circulating form of human uroguanylin [2].

Analytical, diagnostic and therapeutic context of GUCA2B

Northern blot hybridization revealed a high level expression of the GCAP-II gene in human colon, but not in the kidney [4].
In addition, size exclusion chromatography revealed that pro-GCAP-II exists as a dimer in solution [1].

References

Dual function of the propeptide of prouroguanylin in the folding of the mature peptide: disulfide-coupled folding and dimerization. Hidaka, Y., Shimono, C., Ohno, M., Okumura, N., Adermann, K., Forssmann, W.G., Shimonishi, Y. J. Biol. Chem. (2000) [Pubmed]
GCAP-II: isolation and characterization of the circulating form of human uroguanylin. Hess, R., Kuhn, M., Schulz-Knappe, P., Raida, M., Fuchs, M., Klodt, J., Adermann, K., Kaever, V., Cetin, Y., Forssmann, W.G. FEBS Lett. (1995) [Pubmed]
Uroguanylin: gene structure, expression, processing as a peptide hormone, and co-storage with somatostatin in gastrointestinal D-cells. Mägert, H.J., Reinecke, M., David, I., Raab, H.R., Adermann, K., Zucht, H.D., Hill, O., Hess, R., Forssmann, W.G. Regul. Pept. (1998) [Pubmed]
A new human guanylate cyclase-activating peptide (GCAP-II, uroguanylin): precursor cDNA and colonic expression. Hill, O., Cetin, Y., Cieslak, A., Mägert, H.J., Forssmann, W.G. Biochim. Biophys. Acta (1995) [Pubmed]

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