High impact information on hoxa11b

• The main difference in HoxA gene expression between fin and limb development is that in tetrapods (with the exception of newts) Hoxa-11 expression is suppressed by Hoxa-13 in the distal limb bud mesenchyme [1].
• There is, however, a short period of limb bud development where Hoxa-11 and Hoxa-13 overlap similarly to the late expression seen in zebrafish and paddlefish [1].
• In tetrapod limb development Hoxa-11 plays a key role in differentiating the lower limb and Hoxa-13 plays a key role in differentiating the autopodium [1].
• Based on aligned Hoxa-11 amino acid sequences, the protein was demarcated into three segments: Domains I (N-terminal) and III (homeobox + C-terminal), which varied slightly in rates and patterns of evolution, and a variable, overall hydrophilic region (HyD), which partially overlaps with Domain I [2].

Anatomical context of hoxa11b

• We conclude that the early expression pattern in tetrapods is similar to that seen in late fin development and that the local exclusion by Hoxa-13 of Hoxa-11 from the distal limb bud is a derived feature of limb developmental regulation [1].

Other interactions of hoxa11b

• During fin regeneration, hoxa11b and hoxa13b genes are both induced in undifferentiated cells of the distalmost blastema region (DMB) and the proliferating zone (PZ) and later in differentiating bone-forming cells [3].

Analytical, diagnostic and therapeutic context of hoxa11b

• Using electrophoretic mobility shift assays, a 35-bp intron sequence, which is 100% conserved in all Hoxa-11 loci except for the zebrafish Hoxa-11a paralog, was found to bind protein(s) in HeLa and chick whole-cell extracts [2].
• Cloning and sequence analysis of 5' fragment of Hoxa-11 gene in Latimeria chalumnae [4].
• Latimeria Hoxa-11 gene fragment was amplified by PCR, cloned and sequenced [4].

References