Disease relevance of Ung

• 2. There was an approx three-fold increase in the activity of uracil-DNA glycosylase in Morris hepatoma 7800 but there was little change in activity in other hepatomas that were examined [1].

High impact information on Ung

• [14C]Methoxyamine permits one to follow the formation of AP sites in a uracil-containing polydeoxyribonucleotide incubated with calf thymus uracil-DNA glycosylase [2].
• Methoxyamine has no effect on the uracil-DNA glycosylase activity and may be added together with the enzyme in order to block the AP sites and prevent the degradation of the polynucleotide by the AP endonucleases that may be present in a crude preparation [2].
• Uracil-DNA glycosylase from rat liver mitochondria, an inner membrane protein, has been purified approximately 575,000-fold to apparent homogeneity [3].
• Induction of the DNA repair enzymes uracil DNA glycosylase and 3-methyladenine DNA glycosylase in regenerating rat liver [4].
• As measured in nuclear extracts, the specific activities of both the uracil DNA glycosylase and the 3-methyladenine DNA glycosylase were increased in regenerating rat liver reaching maximal levels 18--24 h after partial hepatectomy [4].

Biological context of Ung

• Expression and activity of the DNA repair enzyme uracil DNA glycosylase during organogenesis in the rat conceptus and following methotrexate exposure in vitro [5].

Anatomical context of Ung

• Most of the uracil-DNA glycosylase of the rat liver cell is located in chromatin; there is, however, some activity in the nuclear sap and in the cytoplasm [6].

Associations of Ung with chemical compounds

• Uracil incorporation into DNA occurs under conditions that limit thymidine biosynthesis; uracil is removed by two isoforms of uracil DNA glycosylase (UNG; EC 3.2.2.3), UNG1 and UNG2 [5].
• 3. The activities of dUTPase and uracil-DNA glycosylase were not significantly affected by two diets that may be promotional for hepatocarcinogenesis, a high orotate diet and an arginine-deficient diet [1].

Other interactions of Ung

• dUTP pyrophosphatase and uracil-DNA glycosylase in rat liver and hepatomas [1].

Analytical, diagnostic and therapeutic context of Ung

• In addition, uracil was also detected within the p53 gene sequence using UDG PCR techniques [7].
• Purification of mitochondrial uracil-DNA glycosylase using Ugi-Sepharose affinity chromatography [8].

References