High impact information on UNG1

• Analyses with isogenic uracil-DNA-glycosylase (UNG1) deficient or proficient strains indicate that in the absence of dUTPase, cell death results from the incorporation of uracil into DNA and the attempted repair of this damage by UNG1-mediated excision repair [1].
• Genetic mapping experiments have localized the UNG1 gene on the left arm of chromosome XIII at 17 cM from the GAL80 locus proximal to the centromer [2].
• The gene product was overproduced about 100-fold in strains carrying an UNG1 gene containing plasmid at 100-200 copies/cell [2].
• The DNA repair genes RAD54 and UNG1 have MCB elements in their promoters and are shown to be cell cycle regulated [3].
• Epistasis analyses showed an additive effect between chk1 and ung1, indicating different mechanisms of suppression [4].

Biological context of UNG1

• The DNA repair genes RAD54 and UNG1 are cell cycle regulated in budding yeast but MCB promoter elements have no essential role in the DNA damage response [3].
• All phenotypes of the dut1-1 mutant are suppressed by the simultaneous inactivation of the uracil DNA N-glycosylase, Ung1 [5].
• From this analysis, we conclude that the mutator phenotype of the ung1 deletion strain is the result of a failure to repair spontaneous cytosine deamination events occurring frequently in S. cerevisiae and that the UNG1 gene is not required for strand-specific mismatch repair in S. cerevisiae [6].

Anatomical context of UNG1

• Our study suggests that deamination of cytosine is a frequent event in S.cerevisiae mitochondria and both yeast and human Ung1p repairs deaminated cytosine in mitochondria [7].

Associations of UNG1 with chemical compounds

• Strains containing the ung1 mutation have an increased sensitivity to sodium bisulfite and sodium nitrite but a wild-type sensitivity to methyl methanesulfonate, UV light, and drugs that cause depletion of the thymidylate pool [8].

Other interactions of UNG1

• In yeast mitochondria, three N-glycosylases have been identified so far, Ntg1p, Ogg1p and Ung1p [9].

References