High impact information on tsr

• A similar phenotype was seen in testes treated with cytochalasin B and has been noted previously in mutants at the twinstar locus, a gene that encodes a Drosophila member of the cofilin/ADF family of actin-severing proteins [1].
• Dephosphorylating cofilin appears to be the major function of Slingshot in regulating axon growth in vivo [2].
• Here we show that the actin depolymerization factor cofilin is essential for axon growth in Drosophila neurons [2].
• We find that profilin and cofilin regulate actin-filament formation throughout the cell cortex [3].
• There is also evidence that cofilin/ADF enhances cell motility, although a direct requirement in vivo has not yet been shown [4].

Biological context of tsr

• Cofilin/ADF is required for cell motility during Drosophila ovary development and oogenesis [4].
• Mutations in twinstar, a Drosophila gene encoding a cofilin/ADF homologue, result in defects in centrosome migration and cytokinesis [5].
• We describe the phenotypic and molecular characterization of twinstar (tsr), an essential gene in Drosophila melanogaster [5].
• These findings prompted us to examine actin behavior during male meiosis to visualize the effects of decreased twinstar protein activity on actin dynamics in vivo [5].
• We also show that twinfilin and the ADF/cofilin encoding gene twinstar interact genetically in bristle morphogenesis [6].

Associations of tsr with chemical compounds

• Slingshot cofilin phosphatase localization is regulated by Receptor Tyrosine Kinases and regulates cytoskeletal structure in the developing Drosophila eye [7].

Enzymatic interactions of tsr

• Twinstar/Cofilin becomes phosphorylated upon activation of Mbt [8].
• A Drosophila homolog of LIM-kinase phosphorylates cofilin and induces actin cytoskeletal reorganization [9].

Other interactions of tsr

• Thus, results from a misexpression screen in the eye led us to a putative novel Rac1-Cdi-Cofilin pathway, regulated by RacGAP(84C), coordinating Drosophila spermatogenesis [10].
• These findings, coupled with the analysis of anillin behavior in twinstar mutants, suggested a model on the role of anillin during cytokinesis [11].
• These observations suggest that the LIMK-cofilin signaling pathway for regulating actin filament dynamics is evolutionarily conserved between Drosophila and mammals [9].

References