Disease relevance of RANBP5

• Nonstructural protein 5A of hepatitis C virus inhibits the function of karyopherin beta3 [1].
• Role of ran binding protein 5 in nuclear import and assembly of the influenza virus RNA polymerase complex [2].

High impact information on RANBP5

• Importin beta, transportin, RanBP5 and RanBP7 mediate nuclear import of ribosomal proteins in mammalian cells [3].
• We suggest that karyopherin beta3 is a nuclear transport factor that may mediate the import of some ribosomal proteins into the nucleus [4].
• Karyopherin beta3 was localized mainly to the cytosol and the nucleus, particularly the nuclear rim [4].
• RanBP5 binds to RanBP1 as part of a trimeric RanBP1-Ran-RanBP5 complex [5].
• RanBP5 is a predominantly cytoplasmic protein that can bind to nuclear pore complexes [5].

Biological context of RANBP5

• Like importin-beta, RanBP5 strongly binds the GTP-bound form of Ran, stabilizing it against both intrinsic and RanGAP1-induced GTP hydrolysis and also against nucleotide exchange [5].
• The transmission disequilibrium test (TDT) demonstrated that of these 7 SNPs, rs626716, a T to C base change at the KPNB3 locus, was the only SNP associated with schizophrenia (chi(2) = 7.71, P = 0.005) although the global P-value given by a permutation test was 0.04 for 100 permutations [6].
• The result showed that the KPNB3 haplotypes were also associated with schizophrenia (chi(2) = 10.18, d.f. = 2, P = 0.006) [6].
• Moreover, siRNA knock-down of RanBP5 resulted in the delayed accumulation of viral RNAs in infected cells, confirming that RanBP5 plays a biological role during the influenza virus life cycle [2].
• Recent studies suggest that both the KPNB3 gene and the KPNA3 gene in the long arm of chromosome 13 (13q) are associated with schizophrenia [7].

Anatomical context of RANBP5

• RanBP5 was identified by two independent methods: it was isolated from HeLa cells by using its interaction with RanGTP in an overlay assay to monitor enrichment, and it was also found by the yeast two-hybrid selection method with RanBP1 as bait [5].
• The karyopherin beta3 gene was isolated from a human liver cell library as a protein interacting with NS5A [1].

Associations of RANBP5 with chemical compounds

• The ability of Kluyveromyces marxianus IMB3 to decolorize Remazol Black-B dye was investigated [8].

Other interactions of RANBP5

• Instead, at least four importin beta-like transport receptors, namely importin beta itself, transportin, RanBP5 and RanBP7, directly bind and import ribosomal proteins [3].
• Karyopherin beta2 mediates import of mRNA binding proteins, whereas karyopherin beta3 and beta4 mediate import of a set of ribosomal proteins [9].
• The present work suggests that the combination of the KPNA3 gene and the KPNB3 gene may increase a genetic risk for schizophrenia [7].
• We propose a model in which RanBP5 acts as an import factor for the newly synthesized polymerase by targeting the PB1-PA dimer to the nucleus [2].

References