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Gene Review:

Klp67A - Kinesin-like protein at 67A

Drosophila melanogaster

Synonyms: 0427/05, CG10923, DmKlp67A, Dmel\CG10923, KIF 18A, ...

Pereira, A.J. et al., Goshima, G. et al., Savoian, M.S. et al., Gandhi, R. et al.

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Disease relevance of Klp67A

To prove this directly, KLP67A expressed in Escherichia coli was shown in an in vitro motility assay to move MTs in the plus direction [1].

High impact information on Klp67A

We also report expression analyses at both the mRNA and protein level, which implicate KLP67A in the localization of mitochondria in undifferentiated cell types [1].
DNA sequence analysis of KLP67A predicts an MT motor protein with an amino-terminal motor domain [1].
Furthermore, when affinity-purified anti-KLP67A antisera are used to stain blastoderm embryos, mitochondria in the region of the spindle asters are labeled [1].
In situ hybridization studies of the KLP67A mRNA during embryogenesis and larval central nervous system development indicate a proliferation-specific expression pattern [1].
Class 8 (Klp67A) and class 14 (Ncd) kinesin are sequestered in an active form in the nucleus during interphase and engage their microtubule targets upon nuclear envelope breakdown (NEB) [2].

Biological context of Klp67A

During both mitosis and male meiosis, Klp67A mutations cause an increase in MT length and disrupt discrete aspects of spindle assembly, as well as cytokinesis [3].
Drosophila Klp67A is required for proper chromosome congression and segregation during meiosis I [4].
Consistent with the above abnormalities in chromosome congression, we found that in wild-type cells Klp67A associated with prometaphase/metaphase kinetochores before redistributing to the central spindle at anaphase onset [4].
Klp67A also becomes kinetochore associated upon NEB, but the majority of the population relocalizes to the central spindle by the timing of anaphase A onset, consistent with our RNAi result showing no effect of depleting this motor on anaphase A [2].
Relocalization of Klp67A to the cytoplasm using a nuclear export signal resulted in the disassembly of the interphase microtubule array, providing support for the hypothesis that this kinesin class possesses microtubule-destabilizing activity [2].

Anatomical context of Klp67A

Drosophila Klp67A belongs to the Kip3 subfamily of Kinesin-type microtubule catastrophe factors [4].

References

Mitochondrial association of a plus end-directed microtubule motor expressed during mitosis in Drosophila. Pereira, A.J., Dalby, B., Stewart, R.J., Doxsey, S.J., Goldstein, L.S. J. Cell Biol. (1997) [Pubmed]
Cell cycle-dependent dynamics and regulation of mitotic kinesins in Drosophila S2 cells. Goshima, G., Vale, R.D. Mol. Biol. Cell (2005) [Pubmed]
The Drosophila kinesin-like protein KLP67A is essential for mitotic and male meiotic spindle assembly. Gandhi, R., Bonaccorsi, S., Wentworth, D., Doxsey, S., Gatti, M., Pereira, A. Mol. Biol. Cell (2004) [Pubmed]
Drosophila Klp67A is required for proper chromosome congression and segregation during meiosis I. Savoian, M.S., Gatt, M.K., Riparbelli, M.G., Callaini, G., Glover, D.M. J. Cell. Sci. (2004) [Pubmed]

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