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Gene Review:

ABCC2 - ATP-binding cassette, sub-family C...

Sus scrofa

Fricker, G. et al., Hirouchi, M. et al., Török, M. et al., Magnarin, M. et al.

Fricker, Nobmann, Miller,

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Disease relevance of ABCC2

METHODS: The expression and cellular localization of the wild-type and three kinds of reported SNP variants of MRP2 molecules were analyzed in LLC-PK1 cells after infection with the recombinant Tet-off adenoviruses [1].

High impact information on ABCC2

Conversely, unlabelled octreotide reduced luminal accumulation of fluorescent BODIPY-verapamil on p-glycoprotein and of fluorescein-methotrexate, on Mrp2 [2].
4. Together, the data are consistent with octreotide being transported across the luminal membrane of porcine brain capillaries by both P-gp and Mrp2, providing further evidence that both transporters contribute substantially to the active barrier function of this endothelium [2].
3. The accumulation of NBD-octreotide in capillary lumens was inhibited in a concentration-dependent manner by unlabelled octreotide, by verapamil, PSC-833 and cyclosporin A, potent inhibitors of p-glycoprotein, and by leucotriene C(4), a strong modulator of Mrp2 [2].
RESULTS: The transport activity for E217betaG, LTC4, and DNP-SG, normalized by the expression level of MRP2, was similar between the wild-type, V417I, and A1450T MRP2s [1].
PURPOSE: The presence of single nucleotide polymorphisms (SNPs) has been reported for multidrug resistance-associated protein 2 (MRP2/ABCC2) [1].

Anatomical context of ABCC2

Expression of ABC transporters such as P-glycoprotein, multidrug resistance-associated protein-1(MRP1), and MRP2 were compared by measuring messenger RNA (mRNA) levels in whole-brain tissue, isolated brain capillaries, and cultured cells [3].
In the LLC-PK(1) tubular renal cell line, a 15-day treatment with 25 microM rifampicin significantly increased the mRNA levels of P-glycoprotein, MRP1, MRP2, LRP and cytochrome P450 3A4 (CYP 3A4) [4].

Other interactions of ABCC2

In freshly isolated BCEC, mRNA levels of MRP2 and P-glycoprotein dropped by two- to sevenfold, respectively, whereas MRP1 mRNA levels were slightly increased [3].

Analytical, diagnostic and therapeutic context of ABCC2

During cell culture, mRNA levels of MRP1 and MRP2 decreased by up to fivefold, while P-glycoprotein levels remained constant [3].
Western blot analysis confirmed a moderate increase in the expression of P-glycoprotein and MRP2, but not MRP1 also at the protein level [4].

References

Characterization of the cellular localization, expression level, and function of SNP variants of MRP2/ABCC2. Hirouchi, M., Suzuki, H., Itoda, M., Ozawa, S., Sawada, J., Ieiri, I., Ohtsubo, K., Sugiyama, Y. Pharm. Res. (2004) [Pubmed]
Permeability of porcine blood brain barrier to somatostatin analogues. Fricker, G., Nobmann, S., Miller, D.S. Br. J. Pharmacol. (2002) [Pubmed]
Modulation of transendothelial permeability and expression of ATP-binding cassette transporters in cultured brain capillary endothelial cells by astrocytic factors and cell-culture conditions. Török, M., Huwyler, J., Gutmann, H., Fricker, G., Drewe, J. Experimental brain research. Experimentelle Hirnforschung. Expérimentation cérébrale. (2003) [Pubmed]
Induction of proteins involved in multidrug resistance (P-glycoprotein, MRP1, MRP2, LRP) and of CYP 3A4 by rifampicin in LLC-PK1 cells. Magnarin, M., Morelli, M., Rosati, A., Bartoli, F., Candussio, L., Giraldi, T., Decorti, G. Eur. J. Pharmacol. (2004) [Pubmed]

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