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Gene Review:

CALCR - calcitonin receptor

Sus scrofa

Zolnierowicz, S. et al., Yoshida, N. et al., Lin, H.Y. et al., Kerr, C.A. et al., Teti, A. et al., et al.

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High impact information on CALCR

Expression cloning of an adenylate cyclase-coupled calcitonin receptor [1].
A calcitonin receptor complementary DNA (cDNA) was cloned by expression of a cDNA library from a porcine kidney epithelial cell line in COS cells [1].
To test this hypothesis we studied the LLC-PK1 renal tubular cells and the PC1 cells, a cell line stably transfected with the cloned porcine calcitonin receptor [2].
The gene encoding the calcitonin receptor (CTR) was isolated from a porcine kidney epithelial cell line (LLC-PK1) genomic library and found to span approximately 70 kilobases [3].
In LLC-PK1 cells the mRNA encoding the shorter CTR (CTR-1a) is approximately 1,000 times more abundant than the longer variant (CTR-1b), as estimated by the competitive RT-PCR [3].

Biological context of CALCR

The CTR gene was mapped to chromosome band 9q11-q12 [3].
Isolation, characterization, and chromosomal localization of the porcine calcitonin receptor gene. Identification of two variants of the receptor generated by alternative splicing [3].
The transcription initiation site of the CTR gene was mapped by primer extension, S1 nuclease, and RT-PCR analysis [3].
Analysis of the gene sequence revealed that the CTR mRNA encompasses 14 exons with 12 exons encoding the protein [3].
Transient transfection of CTR gene promoter-luciferase chimeras in LLC-PK1 cells led to the expression of luciferase activity [3].

Associations of CALCR with chemical compounds

The VPR1 mutant cell line possessed less than 5% parental V2 receptor binding for vasopressin but exhibited normal calcitonin receptor binding [4].
By structural analysis, this peptide was determined to be a C-terminal glycine-extended form of calcitonin receptor-stimulating peptide-1 (CRSP-1-Gly) [5].

Other interactions of CALCR

It is known that the calcitonin receptor has dual intracellular signaling pathways, via protein kinases A and C. Both 500 micromol/L 8-bromo-cAMP, a protein kinase A activator, and 100 nmol/L phorbol 12-myristate 13-acetate, a protein kinase C activator, increased CYP27B1 mRNA levels at 24 h (207 +/- 54 and 246 +/- 58% of control, respectively) [6].

Analytical, diagnostic and therapeutic context of CALCR

The expression levels of a novel fragment, '29a,' and the calcitonin receptor gene (CTR) were quantified using quantitative real-time PCR (qRT-PCR) on a subset (n = 709) of the blood samples [7].

References

Expression cloning of an adenylate cyclase-coupled calcitonin receptor. Lin, H.Y., Harris, T.L., Flannery, M.S., Aruffo, A., Kaji, E.H., Gorn, A., Kolakowski, L.F., Lodish, H.F., Goldring, S.R. Science (1991) [Pubmed]
Calcitonin increases cytosolic free calcium concentration via capacitative calcium influx. Teti, A., Paniccia, R., Goldring, S.R. J. Biol. Chem. (1995) [Pubmed]
Isolation, characterization, and chromosomal localization of the porcine calcitonin receptor gene. Identification of two variants of the receptor generated by alternative splicing. Zolnierowicz, S., Cron, P., Solinas-Toldo, S., Fries, R., Lin, H.Y., Hemmings, B.A. J. Biol. Chem. (1994) [Pubmed]
Isolation and genetic characterization of a renal epithelial cell mutant defective in vasopressin (V2) receptor binding and function. Luzius, H., Jans, D.A., Jans, P., Fahrenholz, F. Exp. Cell Res. (1991) [Pubmed]
Isolation and characterization of a glycine-extended form of calcitonin receptor-stimulating peptide-1: another biologically active form of calcitonin receptor-stimulating peptide-1. Katafuchi, T., Hamano, K., Kikumoto, K., Minamino, N. Peptides (2005) [Pubmed]
Calcitonin induces 25-hydroxyvitamin D3 1alpha-hydroxylase mRNA expression via protein kinase C pathway in LLC-PK1 cells. Yoshida, N., Yoshida, T., Nakamura, A., Monkawa, T., Hayashi, M., Saruta, T. J. Am. Soc. Nephrol. (1999) [Pubmed]
The heritability of the expression of two stress-regulated gene fragments in pigs. Kerr, C.A., Bunter, K.L., Seymour, R., Shen, B., Reverter, A. J. Anim. Sci. (2005) [Pubmed]

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