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Gene Review

cysK  -  cysteine synthase A

Escherichia coli UTI89

 
 
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Disease relevance of cysK

 

High impact information on cysK

 

Biological context of cysK

  • Gel mobility shift assays and DNase I footprinting experiments showed that CysB protein binds to the S. typhimurium cysK promoter at two sites, one, designated CBS-K1, at positions -78 to -39 relative to the major transcription start site, and the other, designated CBS-K2, at positions -115 to -79 [2].
  • The encoded CmbR protein is homologous to the LysR family of regulator proteins and is an activator of the metC-cysK operon [3].
  • Other sulfur sources tested showed no significant effect on metC-cysK gene expression [3].
  • After hydrolysis of the purified protein, amino acid sequences of five peptides were obtained, which permitted the cloning and sequencing of the cysK gene [4].
  • The 3.0-kb HindIII-EcoRI fragment bearing cysK contained two additional ORFs encoding a putative transcriptional regulator and dUTPase [4].

References

  1. Isolation of Salmonella typhimurium cys genes by transduction with a library of recombinant plasmids packaged in bacteriophage P22HT capsids. Monroe, R.S., Kredich, N.M. J. Bacteriol. (1988) [Pubmed]
  2. In vitro interactions of CysB protein with the cysK and cysJIH promoter regions of Salmonella typhimurium. Monroe, R.S., Ostrowski, J., Hryniewicz, M.M., Kredich, N.M. J. Bacteriol. (1990) [Pubmed]
  3. Regulation of the metC-cysK operon, involved in sulfur metabolism in Lactococcus lactis. Fernández, M., Kleerebezem, M., Kuipers, O.P., Siezen, R.J., van Kranenburg, R. J. Bacteriol. (2002) [Pubmed]
  4. Identification, cloning and characterization of cysK, the gene encoding O-acetylserine (thiol)-lyase from Azospirillum brasilense, which is involved in tellurite resistance. Ramírez, A., Castañeda, M., Xiqui, M.L., Sosa, A., Baca, B.E. FEMS Microbiol. Lett. (2006) [Pubmed]
  5. Direct detection of potential selenium delivery proteins by using an Escherichia coli strain unable to incorporate selenium from selenite into proteins. Lacourciere, G.M., Levine, R.L., Stadtman, T.C. Proc. Natl. Acad. Sci. U.S.A. (2002) [Pubmed]
  6. Conversion of Methionine to Cysteine in Bacillus subtilis and Its Regulation. Hullo, M.F., Auger, S., Soutourina, O., Barzu, O., Yvon, M., Danchin, A., Martin-Verstraete, I. J. Bacteriol. (2007) [Pubmed]
 
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