High impact information on 14-3-3epsilon

• In epithelia, this complex localizes apically and defines the apical membrane, whereas Bazooka lacking PAR-1 phosphorylation/14-3-3 binding sites forms ectopic lateral complexes [1].
• We show that several Drosophila homologs of checkpoint genes, mei-41, grapes, and 14-3-3epsilon, regulate a DNA damage checkpoint in the developing eye [2].
• The 14-3-3 epsilon protein appears to function in multiple RTK pathways, suggesting that it is a general component of RAS1 signaling cascade [3].
• Our genetic data suggest that 14-3-3 epsilon functions downstream of or parallel to RAF, but upstream of nuclear factors in RAS1 signaling [3].
• We have isolated mutations in the gene encoding a Drosophila 14-3-3 epsilon protein as suppressors of the rough eye phenotype caused by the ectopic expression of RAS1(V12) [3].

Biological context of 14-3-3epsilon

• Dynamic expression and cellular localization of the drosophila 14-3-3epsilon during embryonic development [4].
• Relocalization of 14-3-3 proteins with cell cycle progression suggested cell-cycle-specific roles [5].
• We suggest a model in which 14-3-3 proteins act in the undisturbed cell cycle to set a threshold for entry into mitosis by suppressing Cdk1 activity, to block mitosis following radiation damage and to facilitate proper exit from mitosis [5].
• PAR-1 kinases phosphorylate proteins to generate 14-3-3 binding sites and may therefore directly deliver 14-3-3 to these targets [6].

Anatomical context of 14-3-3epsilon

• In syncytial blastoderm when the nuclei divided rapidly, d14-3-3epsilon localized in the nuclei [4].

Associations of 14-3-3epsilon with chemical compounds

• 14-3-3 proteins form a highly conserved protein family whose members have been shown to activate tyrosine and tryptophan hydroxylases, inhibit protein kinase C and possess phospholipase A2 activity in vitro [7].

Other interactions of 14-3-3epsilon

• Sequence analysis of three dominant-negative alleles defines two regions of 14-3-3 epsilon that participate in RAS1 signaling [3].

Analytical, diagnostic and therapeutic context of 14-3-3epsilon

• Northern blot analysis of poly(A)+ RNA isolated from eight different human tissues shows that 14-3-3 protein mRNAs are expressed in many tissues in the body [7].

References