High impact information on Sry-alpha

• Immunodetection of the sry alpha protein using anti-sry alpha polyclonal antibodies indicates that the 58-kD sry alpha protein accumulates transiently at the blastoderm stage [1].
• P-element rescue experiments show that one copy of the sry alpha gene rescues the defective cellularization phenotype associated with Df(3R)X3F [1].
• The sry alpha protein is associated with the invaginating plasma membrane and colocalizes with F-actin [1].
• Lack of sry alpha activity results in erratic disruptions of the cytoskeleton at the beginning of the interphase of mitotic cycle 14 [1].
• The compared patterns of expression of D. melanogaster sry alpha and nullo, and D. pseudoobscura sry alpha reveal a complex regulation of the spatiotemporal accumulation of their transcripts [2].

Biological context of Sry-alpha

• Sequence comparison of the jan A-sry beta intergenic region and 5'-transcribed domain of each gene between D. melanogaster and D. pseudoobscura reveals short stretches of conserved sequences that may correspond to cis-acting regulator elements [3].
• Blastoderm-specific and read-through transcription of the sry alpha gene transformed into the Drosophila genome [4].
• We present new DNA sequence data on the nullo and Serendipity-alpha genes and combine them with all available nuclear DNA sequence data; the total data encompass 12 genes and the ITS of rDNA [5].
• The Drosophila serendipity (sry) delta (delta) zinc finger protein is a sequence-specific DNA binding protein, maternally inherited by the embryo and present in nuclei of transcriptionally active cells throughout fly development [6].
• We show that a 95 bp sry alpha upstream region is sufficient for blastoderm-specific expression of a lacZ reporter gene [7].

Anatomical context of Sry-alpha

• The Drosophila serendipity alpha (sry alpha) gene is specifically transcribed at the blastoderm stage, from nuclear cycle 11 to the onset of gastrulation, in all somatic nuclei [1].
• The serendipity alpha (sry alpha) gene is required for this complete reorganization of the microfilaments at the onset of membrane invagination [2].
• Despite their functional homologies, the D. melanogaster and D. pseudoobscura sry alpha RNAs have different subcellular localisations, suggesting that this specific localization has no conserved role in targeting the sry alpha protein to the apical membranes [2].

Co-localisations of Sry-alpha

• The serendipity-alpha (sry-alpha) protein co-localizes with nullo protein to the hexagonal network but, unlike the nullo protein, it localizes to the sides rather than the vertices of each hexagon [8].

Regulatory relationships of Sry-alpha

• These two effects depend upon da+ activity, suggesting that daughterless may positively control sry alpha transcription [7].

Analytical, diagnostic and therapeutic context of Sry-alpha

• The sry alpha gene is not itself secondarily expressed in the PNS, as shown by in situ hybridization [9].

References