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Gene Review

EGF  -  epidermal growth factor

Bos taurus

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High impact information on EGF

  • Expression of GFP in skin biopsies isolated from transgenic cloned calves and fibroblasts derived from the skin biopsies revealed the activity of EGFP gene, and G418 resistance in vitro of these fibroblasts confirmed the activity of Neor gene [1].
  • Fetal oviduct epithelial cells (FOECs) were isolated from the oviduct of a Day 147 bovine fetus and transfected with a plasmid (pCE-EGFP-IRES-NEO) containing the enhanced green fluorescent protein (EGFP) and neomycin-resistant (Neor) genes [1].
  • Cells transfected with a plasmid containing the enhanced green fluorescence protein (EGFP) gene, and non-transfected cells were used for cloning between passage 10 and 15 as serum-starved and serum-fed donor cells [2].
  • However, embryonic stem cells could be transduced by BHV-4EGFP deltaTK just after differentiation, as shown by expression of EGFP [3].
  • Nuclear transfer of blastomeres expressing EGFP-reporter gene may improve the efficiency of transgenic cattle [4].

Biological context of EGF

  • The effect of timing of microinjection of DNA constructs on the efficiency of transgenic embryo production and improved efficiency and quality through combining EGFP as a reporter gene with nuclear transfer techniques were examined [4].

Anatomical context of EGF

  • However, developmental competence of DNA-injected zygotes and the EGFP expression rate were not affected by the injection time [4].


  1. Birth of calves expressing the enhanced green fluorescent protein after transfer of fresh or vitrified/thawed blastocysts produced by somatic cell nuclear transfer. Gong, G., Dai, Y., Fan, B., Zhu, H., Zhu, S., Wang, H., Wang, L., Tang, B., Li, R., Wan, R., Liu, Y., Huang, Y., Zhang, L., Sun, X., Li, N. Mol. Reprod. Dev. (2004) [Pubmed]
  2. Production of transgenic bovine embryos by transfer of transfected granulosa cells into enucleated oocytes. Arat, S., Rzucidlo, S.J., Gibbons, J., Miyoshi, K., Stice, S.L. Mol. Reprod. Dev. (2001) [Pubmed]
  3. Interaction of a green recombinant bovine herpesvirus 4 with in vitro-produced bovine embryos. Donofrio, G., Galli, C., Lazzari, G., van Santen, V.L., Cavirani, S., Flammini, C.F. Vet. Res. Commun. (2003) [Pubmed]
  4. Nuclear transfer of blastomeres expressing EGFP-reporter gene may improve the efficiency of transgenic cattle. Funahashi, H., Ideta, A., Konishi, M., Urakawa, M., Uruno, K., Aoyagi, Y., Okabe, M., Niwa, K. Cloning Stem Cells (2001) [Pubmed]
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