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Gene Review

POLR2G  -  polymerase (RNA) II (DNA directed)...

Homo sapiens

Synonyms: DNA-directed RNA polymerase II subunit G, DNA-directed RNA polymerase II subunit RPB7, RNA polymerase II 19 kDa subunit, RNA polymerase II subunit B7, RPB19, ...
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Disease relevance of POLR2G


High impact information on POLR2G

  • Identification of the RNA polymerase II subunit hsRPB7 as a novel target of the von Hippel-Lindau protein [2].
  • In contrast to hsRPB7, hsRPB4 has diverged so that it no longer interacts with yeast RPB7, although it partially complements rpb4- phenotypes in yeast [3].
  • Employing yeast two-hybrid cloning we isolated the seventh largest subunit of human RNA polymerase II (hsRPB7) as a protein that specifically interacts with the amino terminus of EWS [4].
  • Overexpression of recombinant hsRPB7 specifically increased gene activation by EWS-chimeric transcription factors [4].
  • Here, we provide evidence that the Rpb7 subunit of pol II interacts with GAPDH [5].

Biological context of POLR2G


Associations of POLR2G with chemical compounds

  • This protein, hsRPB7, a subunit of RNA polymerase II, interacts with hRARalpha in the absence of RA and addition of RA disrupts the interaction [8].

Physical interactions of POLR2G

  • We have complemented the structural analysis with biochemical studies directed at dissecting the RNA-binding properties of the human Rpb4/Rpb7 complex and that of the homologous E/F complex from Methanocaldococcus jannaschii [9].

Regulatory relationships of POLR2G

  • Finally, surprisingly, hsRPB7 RNA in human cells is expressed in a tissue-specific pattern that differs from that of the RNA polymerase II largest subunit, implying a potential regulatory role for hsRPB7 [10].

Other interactions of POLR2G

  • We have obtained crystals of the human Rpb4/Rpb7 heterodimer and determined the structure to 2.7 A resolution [9].
  • Analysis of the interaction of the novel RNA polymerase II (pol II) subunit hsRPB4 with its partner hsRPB7 and with pol II [3].
  • Replacement of the EWS portion by hsRPB7 in the oncogenic fusion protein restored the transactivating potential of the chimera [4].
  • Two-hybrid screen identified GAPDH as an Rpb7-binding protein [5].

Analytical, diagnostic and therapeutic context of POLR2G


  1. Suprabasal overexpression of the hsRPB7 gene in psoriatic epidermis as identified by a reverse transcriptase-polymerase chain reaction differential display model comparing psoriasis plaque tissue with peritonsillar mucosa. Böckelmann, R., Neugebauer, P., Paseban, N.D., Hüttemann, M., Gollnick, H., Bonnekoh, B. Am. J. Pathol. (2001) [Pubmed]
  2. Identification of the RNA polymerase II subunit hsRPB7 as a novel target of the von Hippel-Lindau protein. Na, X., Duan, H.O., Messing, E.M., Schoen, S.R., Ryan, C.K., di Sant'Agnese, P.A., Golemis, E.A., Wu, G. EMBO J. (2003) [Pubmed]
  3. Analysis of the interaction of the novel RNA polymerase II (pol II) subunit hsRPB4 with its partner hsRPB7 and with pol II. Khazak, V., Estojak, J., Cho, H., Majors, J., Sonoda, G., Testa, J.R., Golemis, E.A. Mol. Cell. Biol. (1998) [Pubmed]
  4. Oncogenic EWS-Fli1 interacts with hsRPB7, a subunit of human RNA polymerase II. Petermann, R., Mossier, B.M., Aryee, D.N., Khazak, V., Golemis, E.A., Kovar, H. Oncogene (1998) [Pubmed]
  5. Glyceraldehyde-3-phosphate dehydrogenase and actin associate with RNA polymerase II and interact with its Rpb7 subunit. Mitsuzawa, H., Kimura, M., Kanda, E., Ishihama, A. FEBS Lett. (2005) [Pubmed]
  6. Human gene for the RNA polymerase II seventh subunit (hsRPB7): structure, expression and chromosomal localization. Schoen, T.J., Chandrasekharappa, S.C., Guru, S.C., Mazuruk, K., Chader, G.J., Rodriguez, I.R. Biochim. Biophys. Acta (1997) [Pubmed]
  7. RNA emerging from the active site of RNA polymerase II interacts with the Rpb7 subunit. Ujvári, A., Luse, D.S. Nat. Struct. Mol. Biol. (2006) [Pubmed]
  8. Ligand-free RAR can interact with the RNA polymerase II subunit hsRPB7 and repress transcription. Shen, X.Q., Bubulya, A., Zhou, X.F., Khazak, V., Golemis, E.A., Shemshedini, L. Endocrine (1999) [Pubmed]
  9. Crystal structure and RNA binding of the Rpb4/Rpb7 subunits of human RNA polymerase II. Meka, H., Werner, F., Cordell, S.C., Onesti, S., Brick, P. Nucleic Acids Res. (2005) [Pubmed]
  10. Human RNA polymerase II subunit hsRPB7 functions in yeast and influences stress survival and cell morphology. Khazak, V., Sadhale, P.P., Woychik, N.A., Brent, R., Golemis, E.A. Mol. Biol. Cell (1995) [Pubmed]
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