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Gene Review

RPB7  -  DNA-directed RNA polymerase II subunit RPB7

Saccharomyces cerevisiae S288c

Synonyms: B16, D9509.22, RNA polymerase II subunit B7, YDR404C
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Disease relevance of RPB7


High impact information on RPB7

  • We have determined the crystal structure of the complex between the Methanococcus jannaschii subunits E and F, the archaeal homologs of RPB7 and RPB4 [6].
  • Loss of the Rpb4/Rpb7 subcomplex in a mutant form of the Rpb6 subunit shared by RNA polymerases I, II, and III [7].
  • Consistent with the growth phenotype, overexpression of RPB7 could suppress the transcriptional defect characteristic of rpb4Delta cells during the mild, but not during a more severe, heat shock [8].
  • We identified a partially sequenced Saccharomyces cerevisiae gene which encodes a protein related to the S. cerevisiae RNA polymerase II subunit, RPB7 [9].
  • C25, like RPB7, is present in submolar ratios, easily dissociates from the enzyme, is essential for cell growth and viability, but is not required in certain transcription assays in vitro [9].

Chemical compound and disease context of RPB7

  • In the syngeneic B16 melanoma tumor model tiprotimod significantly prolonged the medium survival time and reduced the number of visuable metastases in the lungs even when applied after resection of the primary tumor graft [10].
  • Two bacterial strains B16 (Pseudomonas aeruginosa) and DT4 (Pseudomonas sp.) isolated by enrichment technique were found to utilize 2-chlorobenzoic acid (2-Cba) and 4-chlorobenzoic acid (4-Cba) respectively as sole source of carbon and energy [11].

Biological context of RPB7

  • In S.cerevisiae, RPB7 is essential for cell viability while rpb4 null strains are temperature sensitive at low and high temperatures [12].
  • We also show that high expression levels of S.cerevisiae RPB7 and its homologs rescue the sporulation defect of rpb4 homozygous null diploids, but only some of them cause significant enhancement of the pseudohyphal phenotype [12].
  • We showed that in strain HCY53, the mini-Tn3 was inserted at the distal end of an essential gene RPB7, which encodes one of the two subunits, Rpb4-Rbp7, that forms a subcomplex with RNA polymerase II [13].
  • The defective phenotype of strain HCY53 could be corrected by a plasmid bearing the entire RPB7 gene [13].
  • We present a model whereby the stoichiometry of Rpb4 and Rpb7 and their relative levels in the cell play a switch like role in establishing either sporulation or pseudohyphal gene expression [14].

Anatomical context of RPB7


Associations of RPB7 with chemical compounds

  • An allele of the yeast RPB7 gene, encoding an essential subunit of RNA polymerase II, reduces cellular resistance to the antitumor drug bleomycin [13].

Physical interactions of RPB7

  • Use of the yeast two-hybrid analysis in conjunction with computational analysis of the recently reported crystal structure of Rpb4/Rpb7 sub-complex allowed us to identify regions previously not suspected to be involved in the functional interaction of these proteins [17].
  • Saccharomyces cerevisiae Rpb7 also interacted with Nrd1, indicating that the interaction is conserved in evolution [18].

Other interactions of RPB7

  • Whereas RPB7 is essential, RPB4 is dispensable for cellular viability [19].
  • Under certain circumstances, RPB3, RPB4, and RPB7 were cross-linked [20].


  1. Glucan: attempts to demonstrate therapeutic activity against five syngeneic tumors in guinea pigs and mice. Hunter, J.T., Meltzer, M.S., Ribi, E., Fidler, I.J., Hanna, M.G., Zbar, B., Rapp, H.J. J. Natl. Cancer Inst. (1978) [Pubmed]
  2. Clearance of phenylalanine ammonia-lyase from normal and tumor-bearing mice. Shen, R.S., Fritz, R.R., Abell, C.W. Cancer Res. (1977) [Pubmed]
  3. Phenylalanine ammonia-lyase. Induction and purification from yeast and clearance in mammals. Fritz, R.R., Hodgins, D.S., Abell, C.W. J. Biol. Chem. (1976) [Pubmed]
  4. Syntheses and biological activities (topoisomerase inhibition and antitumor and antimicrobial properties) of rebeccamycin analogues bearing modified sugar moieties and substituted on the imide nitrogen with a methyl group. Anizon, F., Belin, L., Moreau, P., Sancelme, M., Voldoire, A., Prudhomme, M., Ollier, M., Sevère, D., Riou, J.F., Bailly, C., Fabbro, D., Meyer, T. J. Med. Chem. (1997) [Pubmed]
  5. Development, physicochemical characterization and preclinical efficacy evaluation of a water soluble glucan sulfate derived from Saccharomyces cerevisiae. Williams, D.L., Pretus, H.A., McNamee, R.B., Jones, E.L., Ensley, H.E., Browder, I.W., Di Luzio, N.R. Immunopharmacology (1991) [Pubmed]
  6. Structure of an archaeal homolog of the eukaryotic RNA polymerase II RPB4/RPB7 complex. Todone, F., Brick, P., Werner, F., Weinzierl, R.O., Onesti, S. Mol. Cell (2001) [Pubmed]
  7. Loss of the Rpb4/Rpb7 subcomplex in a mutant form of the Rpb6 subunit shared by RNA polymerases I, II, and III. Tan, Q., Prysak, M.H., Woychik, N.A. Mol. Cell. Biol. (2003) [Pubmed]
  8. Rpb7 can interact with RNA polymerase II and support transcription during some stresses independently of Rpb4. Sheffer, A., Varon, M., Choder, M. Mol. Cell. Biol. (1999) [Pubmed]
  9. C25, an essential RNA polymerase III subunit related to the RNA polymerase II subunit RPB7. Sadhale, P.P., Woychik, N.A. Mol. Cell. Biol. (1994) [Pubmed]
  10. Immunomodulation by the new synthetic thiazole derivative tiprotimod. 3rd communication: influence on host resistance to microorganisms, tumors and on experimental immune disorders. Schorlemmer, H.U., Dickneite, G., Hänel, H., Dürckheimer, W., Sedlacek, H.H. Arzneimittel-Forschung. (1989) [Pubmed]
  11. Biochemical and genetic studies on degradation of chlorobenzoates by Pseudomonas. Singh, H., Kahlon, R.S. Acta Microbiol. Pol. (1989) [Pubmed]
  12. Domainal organization of the lower eukaryotic homologs of the yeast RNA polymerase II core subunit Rpb7 reflects functional conservation. Singh, S.R., Rekha, N., Pillai, B., Singh, V., Naorem, A., Sampath, V., Srinivasan, N., Sadhale, P.P. Nucleic Acids Res. (2004) [Pubmed]
  13. An allele of the yeast RPB7 gene, encoding an essential subunit of RNA polymerase II, reduces cellular resistance to the antitumor drug bleomycin. He, C.H., Ramotar, D. Biochem. Cell Biol. (1999) [Pubmed]
  14. Relative levels of RNA polII subunits differentially affect starvation response in budding yeast. Singh, S.R., Pillai, B., Balakrishnan, B., Naorem, A., Sadhale, P.P. Biochem. Biophys. Res. Commun. (2007) [Pubmed]
  15. A microtitre cytotoxicity assay useful for the discovery of fermentation-derived antitumor agents. Catino, J.J., Francher, D.M., Edinger, K.J., Stringfellow, D.A. Cancer Chemother. Pharmacol. (1985) [Pubmed]
  16. Nuclear localisation of NOVH protein: a potential role for NOV in the regulation of gene expression. Perbal, B. MP, Mol. Pathol. (1999) [Pubmed]
  17. Mapping the interaction site of Rpb4 and Rpb7 subunits of RNA polymerase II in Saccharomyces cerevisiae. Sareen, A., Choudhry, P., Mehta, S., Sharma, N. Biochem. Biophys. Res. Commun. (2005) [Pubmed]
  18. Rpb7 subunit of RNA polymerase II interacts with an RNA-binding protein involved in processing of transcripts. Mitsuzawa, H., Kanda, E., Ishihama, A. Nucleic Acids Res. (2003) [Pubmed]
  19. Rpb4p is necessary for RNA polymerase II activity at high temperature. Maillet, I., Buhler, J.M., Sentenac, A., Labarre, J. J. Biol. Chem. (1999) [Pubmed]
  20. Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking. Wooddell, C.I., Burgess, R.R. Biochemistry (2000) [Pubmed]
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