High impact information on MICAL1

• The structures of several of these new flavoenzymes have been described, as exemplified by crystallographic analyses of MICAL, histone demethylase LSD1 and tryptophan dehalogenase [1].
• MICAL associates with CasL through this PPKPP sequence [2].
• Through the COOH-terminal region, MICAL also associates with vimentin that is a major component of intermediate filaments [2].
• MICAL is a protein of 118 kDa and is expressed in the thymus, lung, spleen, kidney, testis, and hematopoietic cells [2].
• The MICAL proteins and rab1: a possible link to the cytoskeleton [3]?

Biological context of MICAL1

• In contrast Hb Trout I represents a unique case of slow chemical exchange, which may depend on unusual stereoche mical characteristics of the chloride binding site; chloride binding is pH independent, but shows a significant oxygen linkage, which may be attributed to changes of the lifetime of chloride at the binding site [4].

Anatomical context of MICAL1

• Therefore, the interaction of MICAL-1 and rab1 might provide a link between the Golgi apparatus and the intermediate filament cytoskeleton [5].
• Immunostaining revealed that MICAL localizes along with vimentin intermediate filaments [2].
• Using the microtubule depolymerizing drug nocodazole we detected a link between MICAL-1 and -3, and the microtubule cytoskeleton [3].

Associations of MICAL1 with chemical compounds

• MICAL could act either indirectly, causing a local increase in the concentration of reactive oxygen species (ROS), or directly, inducing redox changes in downstream effectors, such as actin and members of the Rho and Rac family of guanosine triphosphatases [6].

Physical interactions of MICAL1

• These results suggest that MICAL may be a cytoskeletal regulator that connects CasL to intermediate filaments [2].

Co-localisations of MICAL1

• MICAL is a cytoplasmic protein and colocalizes with CasL at the perinuclear area [2].

Other interactions of MICAL1

• MICAL-1 isoforms, novel rab1 interacting proteins [5].

Analytical, diagnostic and therapeutic context of MICAL1

• Cell fractionation revealed that in contrast to the mainly membrane-associated rab1 effector GM130, MICAL-1 displays a predominantly cytosolic localization [5].

References