High impact information on Scpep1

• Cloning of a novel retinoid-inducible serine carboxypeptidase from vascular smooth muscle cells [1].
• The Nf314 cDNA has an open reading frame for a 53-kDa protein 94% similar and 19% identical over 194 amino acid residues to serine carboxypeptidase from yeast cells, barley, and wheat [2].
• Immunoelectron microscopy shows enriched Scpep1 within lysosomes of the PCT, and immunofluorescence microscopy colocalizes Scpep1 with lysosomal-associated membrane protein-2 [3].
• Consistent with previous mRNA studies in kidney, Scpep1 protein is restricted to proximal convoluted tubular epithelium (PCT) [3].
• Immunohistochemical studies demonstrate Scpep1 expression in embryonic heart and vasculature as well as in adult aortic smooth muscle cells and endothelial cells [3].

Anatomical context of Scpep1

• Scpep1 displays a broad expression pattern in adult tissues with detectable levels in epithelia of digestive tract and urinary bladder, islet of Langerhans, type II alveolar cells and macrophages of lung, macrophage-like cells of lymph nodes and spleen, Leydig cells of testis, and nerve fibers in brain and ganglia [3].

Analytical, diagnostic and therapeutic context of Scpep1

• Western blot analysis revealed an immunospecific approximately 35-kDa protein corresponding to endogenous Scpep1 [3].

References