Disease relevance of Renbp

• The recombinant rat RnBP was synthesized in Escherichia coli cells and purified to apparent homogeneity [1].
• The results show that RnBP is not colocalized or coregulated with renin in this model of hypertension [2].

High impact information on Renbp

• Renin-binding protein is expressed mainly in renal tubules and collecting ducts unlike renin, which is expressed in the glomerular afferent arteriole [2].
• Therefore, a physiological stimulus that upregulates renin gene expression in clipped kidneys does not affect RnBP expression [2].
• The activities of CMP-NeuAc synthase, N-acetyl-D-glucosamine 2-epimerase, N-acetyl-D-glucosamine kinase, sialyltransferase and sialidase were similar to or 2-4-fold lower than in liver [3].
• These results indicate that the middle domain of the GlcNAc 2-epimerase molecule participates in the specificity for and binding of nucleotides, and that nucleotides are essential to form the catalytic domain of the enzyme [4].
• Rat gene for renin-binding protein (RnBP) was shown to be expressed in the kidney, adrenal gland, brain, lung, spleen, ovary, testis, and heart [1].

Biological context of Renbp

• Tissue-specific regulation of renin-binding protein gene expression in rats [1].
• The RnBP mRNAs were expressed in the kidneys as the same size of 1.5-kb and the genes are suggested to exist as single copies in the genomes [5].
• The presence of a leucine zipper motif was recognized in the deduced amino acid sequences of human and rat renin-binding proteins (RnBPs) on cloning and sequence analysis of the RnBP cDNAs [5].
• These results suggest that the expression of RnBP gene in the kidney and adrenal gland is regulated independently, and the function of RnBP is related to electrolyte homeostasis, probably through the interaction with renin [1].
• Assignment of the gene encoding renin binding protein (Renbp) to rat chromosome Xq37 by in situ hybridization and radiation hybrid mapping [6].

Associations of Renbp with chemical compounds

• On sodium depletion and captopril administration, the rat showed a marked increase in the adrenal RnBP mRNA level and a slight decrease in the kidney RnBP mRNA level [1].

Other interactions of Renbp

• Intravenous injection of the RnBP into rats resulted in a rapid and strong inhibition of plasma renin activity, which persisted at least for 2 h [1].

Analytical, diagnostic and therapeutic context of Renbp

• In situ hybridization for renin mRNA was carried out using digoxygenin-labelled antisense oligonucleotides and for RnBP using labelled antisense oligonucleotides and an antisense riboprobe [2].

References