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Gene Review:

KOB1 - protein elongation defective 1

Arabidopsis thaliana

Synonyms: ABA INSENSITIVE 8, ABI8, ELD1, ELONGATION DEFECTIVE 1, KOBITO

Lertpiriyapong, K. et al., Pagant, S. et al., Brocard-Gifford, I. et al., Fang, Z. et al., Cheng, J.C. et al.

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Disease relevance of KOB1

Studies of genetic interactions demonstrate that ABA hypersensitivity conferred by the ABA-hypersensitive1 mutation or overexpression of ABI3 or ABI5 does not suppress the dwarfing and Glc dependence caused by abi8 but partially suppresses ABA-resistant germination [1].

High impact information on KOB1

By contrast, the ABA-insensitive8 (abi8) mutant, isolated on the basis of ABA-resistant germination, also displays severely stunted growth, defective stomatal regulation, altered ABA-responsive gene expression, delayed flowering, and male sterility [1].
KOB1 was cloned and encodes a novel, highly conserved, plant-specific protein that is plasma membrane bound, as shown with a green fluorescent protein-KOB1 fusion protein [2].
Interestingly, in the meristematic zone of kob1 roots, microfibrils appeared unaltered compared with the wild type, suggesting a role for KOB1 preferentially in rapidly elongating cells [2].
The dwarf phenotype of eld1 could not be rescued by treatment with exogenous growth regulators [3].
However, based on ELD1-GFP localization in plasmolyzed cells, we found that ELD1 is localized to the cell wall/extracellular matrix, rather than the membrane [4].

Biological context of KOB1

To investigate the relationship between ELD1 and Arabidopsis genes with high sequence similarity, we analyzed the possible subcellular location of their proteins as well as their amino acid sequence [4].
Thus, ELD1/KOB1 is a secreted protein involved in promoting cell growth [4].
Using reverse genetics, we identified a new allele, eld1-2, which is phenotypically indistinguishable from eld1-1, but does not produce a detectable ELD1 transcript [4].

Anatomical context of KOB1

In contrast to the wild type, which showed transversely oriented parallel microfibrils, kob1 microfibrils were randomized and occluded by a layer of pectic material [2].

Associations of KOB1 with chemical compounds

Using the elongation defective 1-1 (eld1-1) mutant, we cloned the ELD1 gene, which encodes a serine-rich protein [4].

Other interactions of KOB1

The rice cDNA (KOB1) described in this report encodes a 36 kDa polypeptide which shares 45% sequence identity with these animal K+ channel beta subunits. and 72% identity with the only other cloned plant (Arabidopsis thaliana) K+ channel beta subunit (KAB1) [5].

References

The Arabidopsis thaliana ABSCISIC ACID-INSENSITIVE8 encodes a novel protein mediating abscisic acid and sugar responses essential for growth. Brocard-Gifford, I., Lynch, T.J., Garcia, M.E., Malhotra, B., Finkelstein, R.R. Plant Cell (2004) [Pubmed]
KOBITO1 encodes a novel plasma membrane protein necessary for normal synthesis of cellulose during cell expansion in Arabidopsis. Pagant, S., Bichet, A., Sugimoto, K., Lerouxel, O., Desprez, T., McCann, M., Lerouge, P., Vernhettes, S., Höfte, H. Plant Cell (2002) [Pubmed]
The role of the Arabidopsis ELD1 gene in cell development and photomorphogenesis in darkness. Cheng, J.C., Lertpiriyapong, K., Wang, S., Sung, Z.R. Plant Physiol. (2000) [Pubmed]
The elongation defective1 mutant of Arabidopsis is impaired in the gene encoding a serine-rich secreted protein. Lertpiriyapong, K., Sung, Z.R. Plant Mol. Biol. (2003) [Pubmed]
Molecular cloning and expression characterization of a rice K+ channel beta subunit. Fang, Z., Kamasani, U., Berkowitz, G.A. Plant Mol. Biol. (1998) [Pubmed]

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