High impact information on HAT1

• Thus, the HAT4 gene of Arabidopsis encodes an HD-Zip protein that functions as a novel developmental regulator [1].
• The REVOLUTA subfamily of HD-ZIP transcription factors controls the organization of lateral meristems [2].
• Radial patterning of Arabidopsis shoots by class III HD-ZIP and KANADI genes [3].
• In Arabidopsis, gain-of-function alleles of PHAVOLUTA and PHABULOSA, members of the class III HD-ZIP gene family, result in adaxialization of lateral organs [3].
• Genetic analysis revealed that the hat1 phenotype is caused by a dominant mutation at a single nuclear locus [4].

Biological context of HAT1

• CONCLUSIONS: Based on complementary vascular and leaf phenotypes of class III HD-ZIP and KANADI mutants, we propose that a common genetic program dependent upon miRNAs governs adaxial-abaxial patterning of leaves and radial patterning of stems in the angiosperm shoot [3].
• Mapping studies indicate that Hat1 is located at an interval of 168 kb on chromosome 5 in which 21 genes are known to be regulated by diverse abiotic stresses [4].
• The HD-Zip-9 domain recognizes a 11 bp pseudopalindromic sequence (GTAAT(G/C)ATTAC), as determined by selecting high-affinity binding sites from random-sequence DNA [5].

Associations of HAT1 with chemical compounds

• Moreover, gel retardation assays demonstrated that the HD-Zip-9 domain binds to DNA as a dimer [5].

Other interactions of HAT1

• RESULTS: We show that gain-of-function alleles of REVOLUTA, another member of the class III HD-ZIP gene family, are characterized by adaxialized lateral organs and alterations in the radial patterning of vascular bundles in the stem [3].
• This gene encodes a class I HD-Zip protein, and constitutes a gene subfamily with the Daucus carota gene CHB6, and Arabidopsis thaliana genes Athb-5, Athb-6, and Athb-16 [6].
• Athb-8, -9 and -14 are members of a small family of HD-Zip proteins (HD-ZIP III) characterized by a HD-Zip motif confined to the N-terminus of the polypeptide [5].

Analytical, diagnostic and therapeutic context of HAT1

• Two-hybrid assays showed that Oshox1 protein can homodimerize, but can also form heterodimers with an Arabidopsis HD-Zip protein [7].

References