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Gene Review:

TPST2 - tyrosylprotein sulfotransferase 2

Homo sapiens

Synonyms: Protein-tyrosine sulfotransferase 2, TANGO13B, TPST-2, Tyrosylprotein sulfotransferase 2

Hoffhines, A.J. et al., Beisswanger, R. et al., Mishiro, E. et al., Goettsch, S. et al., Nishimura, M. et al.

Nishimura, Imai, Morioka, Kuribayashi, Kamataki, Naito,

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Disease relevance of TPST2

This indicates that TPST1 and TPST2 have distinct macromolecular substrate specificities and provides clues as to the molecular mechanism of the infertility of Tpst2(-/-) males [1].

High impact information on TPST2

Existence of distinct tyrosylprotein sulfotransferase genes: molecular characterization of tyrosylprotein sulfotransferase-2 [2].
A 304-residue segment in the luminal domain of TPST-2 shows 75% amino acid identity to the corresponding segment of TPST-1, including conservation of the residues implicated in the binding of PAPS [2].
Human TPST-2 is a type II transmembrane protein of 377 aa residues that is encoded by a ubiquitously expressed 1.9-kb mRNA originating from seven exons of a gene located on chromosome 22 (22q12.1) [2].
Protein tyrosine O-sulfation is a post-translational modification mediated by one of two Golgi tyrosylprotein sulfotransferases (TPST1 and TPST2) that catalyze the transfer of sulfate to tyrosine residues in secreted and transmembrane proteins [1].
Sustained application of arterial laminar shear stress causes downregulation of TPST1 mRNA and protein expression, while TPST2 is upregulated [3].

Biological context of TPST2

Shear stress-dependent downregulation of TPST1 involves tyrosine kinase, while upregulation of TPST2 is mediated by a protein kinase C-dependent pathway [corrected] [3].

Associations of TPST2 with chemical compounds

CHST3, CHST4 and TPST2 mRNA levels after exposure to 50 microM NO-1886 were not significantly increased [4].
Additionally, the activity of TPST-2, but not TPST-1, was stimulated in the presence of Mg(2+) [5].

Analytical, diagnostic and therapeutic context of TPST2

Quantitative real-time PCR analysis showed that although the two TPSTs were co-expressed in all 20 human tissues examined, the levels of expression of TPST-1 and TPST-2 varied significantly among different tissues [5].
Finally, Western blot analysis using PSG2 showed that certain sperm/epididymal proteins are undersulfated in Tpst2(-/-) mice [1].

References

Detection and Purification of Tyrosine-sulfated Proteins Using a Novel Anti-sulfotyrosine Monoclonal Antibody. Hoffhines, A.J., Damoc, E., Bridges, K.G., Leary, J.A., Moore, K.L. J. Biol. Chem. (2006) [Pubmed]
Existence of distinct tyrosylprotein sulfotransferase genes: molecular characterization of tyrosylprotein sulfotransferase-2. Beisswanger, R., Corbeil, D., Vannier, C., Thiele, C., Dohrmann, U., Kellner, R., Ashman, K., Niehrs, C., Huttner, W.B. Proc. Natl. Acad. Sci. U.S.A. (1998) [Pubmed]
Shear stress mediates tyrosylprotein sulfotransferase isoform shift in human endothelial cells. Goettsch, S., Goettsch, W., Morawietz, H., Bayer, P. Biochem. Biophys. Res. Commun. (2002) [Pubmed]
Effects of NO-1886 (Ibrolipim), a lipoprotein lipase-promoting agent, on gene induction of cytochrome P450s, carboxylesterases, and sulfotransferases in primary cultures of human hepatocytes. Nishimura, M., Imai, T., Morioka, Y., Kuribayashi, S., Kamataki, T., Naito, S. Drug Metab. Pharmacokinet. (2004) [Pubmed]
Differential Enzymatic Characteristics and Tissue-Specific Expression of Human TPST-1 and TPST-2. Mishiro, E., Sakakibara, Y., Liu, M.C., Suiko, M. J. Biochem. (2006) [Pubmed]

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