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Gene Review:

PUF3 - Puf3p

Saccharomyces cerevisiae S288c

Synonyms: L1325, Pumilio homology domain family member 3, YLL013C, mRNA-binding protein PUF3

García-Rodríguez, L.J. et al., Purnelle, B. et al., Olivas, W. et al., Houshmandi, S.S. et al., Jackson, J.S. et al., et al.

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High impact information on PUF3

In addition, Puf3p links the mitochore, a complex required for mitochondrial-cytoskeletal interactions, to the Arp2/3 complex, the force generator for actin-dependent, bud-directed mitochondrial movement [1].
These findings support a role for Puf3p in mitochondrial biogenesis through effects on mRNA interactions [1].
Overexpression of PUF3 results in reduced mitochondrial respiratory activity and reduced levels of Pet123p, a protein encoded by a Puf3p-binding mRNA [1].
Puf3p, a Pumilio family RNA binding protein, localizes to mitochondria and regulates mitochondrial biogenesis and motility in budding yeast [1].
Thus, Puf3p is a mitochondrial protein that contributes to the biogenesis and motility of the organelle [1].

Biological context of PUF3

The Puf3 protein is a transcript-specific regulator of mRNA degradation in yeast [2].
We have investigated the sequences required for Puf3p recruitment to this 3' UTR and have identified two separate binding sites [3].
Since our nucleotide sequence overlaps by 11.6 kb the cosmid 2B18 sequenced by Miosga and Zimmerman (1996) on the right end, we have not reported here the analysis of the ORFs L1313, L1321 and L1325 [4].
The analysis of a 32 kb DNA fragment from cosmid 2G12 on the left arm of chromosome XII identifies 14 open reading frames (ORFs) numbered L0948 to L1325, a new tRNA for proline, a delta remnant and two putative ARS [4].

Physical interactions of PUF3

Puf3p also binds to the COX17 mRNA 3'-UTR in vitro [2].
Each of these amino acid changes in Puf3p enhanced binding to the Puf5p target HO RNA, suggesting a different mode of binding to this target [5].

Regulatory relationships of PUF3

Here we study the mechanism by which the yeast Puf3p binds and stimulates the degradation of COX17 mRNA [5].

Other interactions of PUF3

Examination of COX17 mRNA indicates that Puf3p specifically promotes decay of this mRNA by enhancing the rate of deadenylation and subsequent turnover [2].
We identified distinct sequence motifs in the 3'-untranslated regions of the mRNAs bound by Puf3p, Puf4p, and Puf5p [6].
In addition, the rapid deadenylation of the COX17 mRNA, which is controlled by a member of the Pumilio family of deadenylation activators Puf3p, requires an active Ccr4p/Pop2p/Not deadenylase [7].
These sites are specific for Puf3p, as they cannot bind another Puf protein, Puf5p [3].

References

Puf3p, a Pumilio family RNA binding protein, localizes to mitochondria and regulates mitochondrial biogenesis and motility in budding yeast. García-Rodríguez, L.J., Gay, A.C., Pon, L.A. J. Cell Biol. (2007) [Pubmed]
The Puf3 protein is a transcript-specific regulator of mRNA degradation in yeast. Olivas, W., Parker, R. EMBO J. (2000) [Pubmed]
Recruitment of the Puf3 protein to its mRNA target for regulation of mRNA decay in yeast. Jackson, J.S., Houshmandi, S.S., Lopez Leban, F., Olivas, W.M. RNA (2004) [Pubmed]
The sequence of 32b on the left arm of yeast chromosome XII reveals six known genes, a new member of the seripauperins family and a new ABS transporter homologous to the human multidrug resistance protein. Purnelle, B., Goffeau, A. Yeast (1997) [Pubmed]
Yeast Puf3 mutants reveal the complexity of Puf-RNA binding and identify a loop required for regulation of mRNA decay. Houshmandi, S.S., Olivas, W.M. RNA (2005) [Pubmed]
Extensive association of functionally and cytotopically related mRNAs with Puf family RNA-binding proteins in yeast. Gerber, A.P., Herschlag, D., Brown, P.O. PLoS Biol. (2004) [Pubmed]
Ccr4p is the catalytic subunit of a Ccr4p/Pop2p/Notp mRNA deadenylase complex in Saccharomyces cerevisiae. Tucker, M., Staples, R.R., Valencia-Sanchez, M.A., Muhlrad, D., Parker, R. EMBO J. (2002) [Pubmed]

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