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Gene Review

MPT5  -  Mpt5p

Saccharomyces cerevisiae S288c

Synonyms: BIC834, HTR1, PUF5, Protein HTR1, Pumilio homology domain family member 5, ...
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High impact information on MPT5

  • Furthermore, this relocalization is dependent on both UTH4 a novel yeast gene that extends life span, and its homologue YGL023 [1].
  • Loss of MPT5 increased expression of reporter genes integrated into the ho locus, whereas overexpression of MPT5 decreased expression [2].
  • Here we report that Mpt5 negatively regulates expression of the HO gene [2].
  • Budding yeast, Saccharomyces cerevisiae, has five proteins with conserved Puf motifs: Mpt5/Uth4, Ygl014w, Yll013c, Jsn1, and Ypr042c [2].
  • An interaction between Mpt5p and Cdc28p was detected [3].

Biological context of MPT5

  • Loss of Mpt5p results in phenotypes associated with a weakened cell wall, including sorbitol-remedial temperature sensitivity and sensitivities to calcofluor white and sodium dodecyl sulfate [4].
  • ORF biC834 shows 100% bp identity with the recently identified multicopy suppressor gene of the pop2 mutation (MPT5); its deduced protein product carries an eight-repeat domain region, homologous to that found in the hypothetical regulatory YGL023 protein of S. cerevisiae and the Pumilio protein of Drosophila [5].
  • Throughout the range of pheromone concentrations sufficient to cause cell cycle arrest, Gpa1 stimulates adaptive mechanisms that are partially dependent on Msg5 and Mpt5 [6].
  • The gene could also suppress the temperature sensitivity of the htr1 disruptant (Kikuchi et al. (1994) Mol. Gen. Genet. 245, 107-116) and was physically mapped in the region near PEP3 on chromosome XII R [7].
  • A new gene (SMS1; serine-rich multi-copy suppressor) of Saccharomyces cerevisiae was isolated by the ability to suppress the temperature sensitivity of the htr1 disruptant (Kikuchi et al. (1994) Mol. Gen. Genetics, in press) by increasing its gene dosage [8].

Anatomical context of MPT5


Associations of MPT5 with chemical compounds


Physical interactions of MPT5

  • Studies using a three-hybrid assay for RNA binding indicate that Mpt5 binds to the 3'-UTR of HO mRNA containing a UUGU sequence but not a UACU sequence [2].

Regulatory relationships of MPT5


Other interactions of MPT5

  • Saccharomyces cerevisiae MPT5 and SSD1 function in parallel pathways to promote cell wall integrity [4].
  • These analyses have defined three genetic pathways that function in parallel to promote cell integrity: an Mpt5p-containing pathway, an Ssd1p-containing pathway, and a Pkc1p-dependent pathway [4].
  • Additionally, we find that mutation of MPT5, in the absence of SSD1-V, is lethal in combination with loss of either Ccr4p or Swi4p [4].
  • Mutation of UUGU to UACU in the HO 3'-UTR abolished Mpt5-mediated repression [2].
  • These sites are specific for Puf3p, as they cannot bind another Puf protein, Puf5p [10].


  1. Redistribution of silencing proteins from telomeres to the nucleolus is associated with extension of life span in S. cerevisiae. Kennedy, B.K., Gotta, M., Sinclair, D.A., Mills, K., McNabb, D.S., Murthy, M., Pak, S.M., Laroche, T., Gasser, S.M., Guarente, L. Cell (1997) [Pubmed]
  2. Post-transcriptional regulation through the HO 3'-UTR by Mpt5, a yeast homolog of Pumilio and FBF. Tadauchi, T., Matsumoto, K., Herskowitz, I., Irie, K. EMBO J. (2001) [Pubmed]
  3. Saccharomyces cerevisiae Mpt5p interacts with Sst2p and plays roles in pheromone sensitivity and recovery from pheromone arrest. Chen, T., Kurjan, J. Mol. Cell. Biol. (1997) [Pubmed]
  4. Saccharomyces cerevisiae MPT5 and SSD1 function in parallel pathways to promote cell wall integrity. Kaeberlein, M., Guarente, L. Genetics (2002) [Pubmed]
  5. The DNA sequence of a 7941 bp fragment of the left arm of chromosome VII of Saccharomyces cerevisiae contains four open reading frames including the multicopy suppressor gene of the pop2 mutation and a putative serine/threonine protein kinase gene. Coglievina, M., Bertani, I., Klima, R., Zaccaria, P., Bruschi, C.V. Yeast (1995) [Pubmed]
  6. The yeast pheromone-responsive G alpha protein stimulates recovery from chronic pheromone treatment by two mechanisms that are activated at distinct levels of stimulus. Zhou, J., Arora, M., Stone, D.E. Cell Biochem. Biophys. (1999) [Pubmed]
  7. A high dose of the STM1 gene suppresses the temperature sensitivity of the tom1 and htr1 mutants in Saccharomyces cerevisiae. Utsugi, T., Toh-e, A., Kikuchi, Y. Biochim. Biophys. Acta (1995) [Pubmed]
  8. The SMS1 gene encoding a serine-rich transmembrane protein suppresses the temperature sensitivity of the htr1 disruptant in Saccharomyces cerevisiae. Takeuchi, J., Okada, M., Toh-e, A., Kikuchi, Y. Biochim. Biophys. Acta (1995) [Pubmed]
  9. The HTR1 gene is a dominant negative mutant allele of MTH1 and blocks Snf3- and Rgt2-dependent glucose signaling in yeast. Schulte, F., Wieczorke, R., Hollenberg, C.P., Boles, E. J. Bacteriol. (2000) [Pubmed]
  10. Recruitment of the Puf3 protein to its mRNA target for regulation of mRNA decay in yeast. Jackson, J.S., Houshmandi, S.S., Lopez Leban, F., Olivas, W.M. RNA (2004) [Pubmed]
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