The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

SSL1  -  TFIIH/NER complex subunit SSL1

Saccharomyces cerevisiae S288c

Synonyms: General transcription and DNA repair factor IIH subunit SSL1, Suppressor of stem-loop protein 1, TFIIH subunit SSL1, YLR005W
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

High impact information on SSL1

  • The 85 kd and 50 kd subunits of factor b are now identified as RAD3 and SSL1 proteins, respectively; both are known to be involved in DNA repair [1].
  • SSL1, a suppressor of a HIS4 5'-UTR stem-loop mutation, is essential for translation initiation and affects UV resistance in yeast [2].
  • In light of the related effects of mutations in the SSL1 and SSL2 genes, the encoded proteins may functionally interact both to promote DNA repair and perform an essential function during translation initiation [2].
  • SSL1 suppressor mutants that are conditional for growth have altered polysome profiles at the restrictive temperature, and their cell-free extracts are thermolabile in their ability to translate exogenously added mRNA [2].
  • SSL1 encodes an essential protein of 52 kD with features characteristic of a protein with multiple zinc fingers [2].

Biological context of SSL1

  • Surprisingly, SSL1 suppressor mutations that are shown to confer an in vivo and in vitro defect in translation initiation also rendered yeast hypersensitive to UV irradiation [2].
  • In double mutants, these effects are suppressed, indicating that the RAD3 and SSL1 gene products work together in influencing genome stability [3].
  • Contrary to the case of SSL1, which is an essential gene of S. cerevisiae, p47 is not essential for the viability of Sz. pombe [4].
  • Transcription analysis revealed that the SSL1 gene is transcribed only in MAT alpha cells but not in MAT alpha or diploid (MAT alpha/MAT alpha) cells defining it as a member of the set of alpha-specific genes [5].
  • The SSL1 gene of S. cerevisiae which is involved in the cell-type specific recovery of mating type alpha cells from cell cycle arrest by the mating hormone a-factor has been cloned as a 1 kb Sau3A fragment in a complementing plasmid from a library of S. cerevisae genomic DNA [5].

Associations of SSL1 with chemical compounds

  • The latter motif consists of five cysteine residues and is also present in hp44, SSL1 and another TFIIH subunit, human p34 (hp34) [4].

Physical interactions of SSL1

  • SSL1 protein interacts with itself and with RAD3 and TFB1 proteins in living yeast cells [6].

Other interactions of SSL1

  • The essential TFB1 and SSL1 genes of the yeast Saccharomyces cerevisiae encode two subunits of the RNA polymerase II transcription factor TFIIH (factor b) [7].


  1. Dual roles of a multiprotein complex from S. cerevisiae in transcription and DNA repair. Feaver, W.J., Svejstrup, J.Q., Bardwell, L., Bardwell, A.J., Buratowski, S., Gulyas, K.D., Donahue, T.F., Friedberg, E.C., Kornberg, R.D. Cell (1993) [Pubmed]
  2. SSL1, a suppressor of a HIS4 5'-UTR stem-loop mutation, is essential for translation initiation and affects UV resistance in yeast. Yoon, H., Miller, S.P., Pabich, E.K., Donahue, T.F. Genes Dev. (1992) [Pubmed]
  3. Novel mutations in the RAD3 and SSL1 genes perturb genome stability by stimulating recombination between short repeats in Saccharomyces cerevisiae. Maines, S., Negritto, M.C., Wu, X., Manthey, G.M., Bailis, A.M. Genetics (1998) [Pubmed]
  4. Analysis of TFIIH subunit through isolation of the gene from Schizosaccharomyces pombe corresponding to that of Saccharomyces cerevisiae SSL1, reveals the presence of conserved structural motifs. Adachi, N., Matsumoto, M., Hasegawa, S., Yamamoto, T., Horikoshi, M. Yeast (1999) [Pubmed]
  5. Transcriptional control of SSL1, a gene controlling alpha-specific inactivation of a-factor in Saccharomyces cerevisiae. Steden, M., Duntze, W. FEMS Microbiol. Lett. (1990) [Pubmed]
  6. Yeast RAD3 protein binds directly to both SSL2 and SSL1 proteins: implications for the structure and function of transcription/repair factor b. Bardwell, L., Bardwell, A.J., Feaver, W.J., Svejstrup, J.Q., Kornberg, R.D., Friedberg, E.C. Proc. Natl. Acad. Sci. U.S.A. (1994) [Pubmed]
  7. The yeast TFB1 and SSL1 genes, which encode subunits of transcription factor IIH, are required for nucleotide excision repair and RNA polymerase II transcription. Wang, Z., Buratowski, S., Svejstrup, J.Q., Feaver, W.J., Wu, X., Kornberg, R.D., Donahue, T.F., Friedberg, E.C. Mol. Cell. Biol. (1995) [Pubmed]
WikiGenes - Universities