The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

SSL2  -  TFIIH/NER complex ATPase/helicase subunit...

Saccharomyces cerevisiae S288c

Synonyms: DNA repair helicase RAD25, General transcription and DNA repair factor IIH subunit RAD25, LOM3, RAD25, Suppressor of stem-loop mutation 2, ...
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of SSL2


High impact information on SSL2


Biological context of SSL2

  • We present genetic evidence suggesting that the DNA-repair function of SSL2 protein is not dependent on its essential function [5].
  • It was established that the uvs112 mutation caused a 2.5-fold reduction in the number of recombinants produced by conversion and also significantly increased the frequency of mitotic crossing-over in interplasmid recombination [6].
  • Tetrad analysis placed the UVS112 gene on the left arm of chromosome IX, approximately 20 cM from HIS5 [6].
  • The SSL2 protein has a high degree of amino acid sequence identity to the protein encoded by the human ERCC3 gene (Gulyas, K. D., and Donahue, T. F. (1992) Cell 69, 1031-0142) [7].
  • We examined several yeast strains with different mutations in the essential SSL2 (Suppressor of Stem Loop, also called RAD25) gene for their ability to remove cyclobutane pyrimidine dimers from expressed genes, and from the genome overall [7].

Associations of SSL2 with chemical compounds

  • DNA unwinding was probed with an in vivo permanganate reactivity assay, in a temperature-sensitive mutant of RAD25 [8].
  • However, the role of Ssl2 (Rad25) in cisplatin sensitivity has not been examined [9].

Physical interactions of SSL2


Regulatory relationships of SSL2

  • Multiple mutant alleles of SSL2 exhibited a pronounced growth defect when co-expressed with a mutant allele of Hsp90 [10].

Other interactions of SSL2


  1. RAD25 (SSL2), the yeast homolog of the human xeroderma pigmentosum group B DNA repair gene, is essential for viability. Park, E., Guzder, S.N., Koken, M.H., Jaspers-Dekker, I., Weeda, G., Hoeijmakers, J.H., Prakash, S., Prakash, L. Proc. Natl. Acad. Sci. U.S.A. (1992) [Pubmed]
  2. Dual roles of a multiprotein complex from S. cerevisiae in transcription and DNA repair. Feaver, W.J., Svejstrup, J.Q., Bardwell, L., Bardwell, A.J., Buratowski, S., Gulyas, K.D., Donahue, T.F., Friedberg, E.C., Kornberg, R.D. Cell (1993) [Pubmed]
  3. SSL2, a suppressor of a stem-loop mutation in the HIS4 leader encodes the yeast homolog of human ERCC-3. Gulyas, K.D., Donahue, T.F. Cell (1992) [Pubmed]
  4. RAD25 is a DNA helicase required for DNA repair and RNA polymerase II transcription. Guzder, S.N., Sung, P., Bailly, V., Prakash, L., Prakash, S. Nature (1994) [Pubmed]
  5. Yeast RAD3 protein binds directly to both SSL2 and SSL1 proteins: implications for the structure and function of transcription/repair factor b. Bardwell, L., Bardwell, A.J., Feaver, W.J., Svejstrup, J.Q., Kornberg, R.D., Friedberg, E.C. Proc. Natl. Acad. Sci. U.S.A. (1994) [Pubmed]
  6. UVS112--A gene involved in excision repair of yeast. Kozhina, T., Kozhin, S., Stepanova, V., Yarovoy, B., Donich, V., Fedorova, I., Korolev, V. Yeast (1995) [Pubmed]
  7. The COOH terminus of suppressor of stem loop (SSL2/RAD25) in yeast is essential for overall genomic excision repair and transcription-coupled repair. Sweder, K.S., Hanawalt, P.C. J. Biol. Chem. (1994) [Pubmed]
  8. Rad25p, a DNA helicase subunit of yeast transcription factor TFIIH, is required for promoter escape in vivo. Ostapenko, D., Gileadi, O. Gene (2000) [Pubmed]
  9. The requirement of yeast Ssl2 (Rad25) for the repair of cisplatin-damaged DNA. Yang, W.L., Cvijic, M.E., Ishii, K., Chin, K.V. Biochem. Biophys. Res. Commun. (1998) [Pubmed]
  10. Novel interaction of the Hsp90 chaperone machine with Ssl2, an essential DNA helicase in Saccharomyces cerevisiae. Flom, G., Weekes, J., Johnson, J.L. Curr. Genet. (2005) [Pubmed]
  11. The Candida albicans 14-3-3 gene, BMH1, is essential for growth. Cognetti, D., Davis, D., Sturtevant, J. Yeast (2002) [Pubmed]
  12. Transcription factor TFIIH is required for promoter melting in vivo. Guzmán, E., Lis, J.T. Mol. Cell. Biol. (1999) [Pubmed]
WikiGenes - Universities