High impact information on KIN3

• These results suggest the existence of a MAPK cascade that consists of NPK1, NQK1/NtMEK1, and NRK1 and functions in a process related to the architecture of phragmoplasts at the late M phase of the cell cycle [1].
• Both NQK1/NtMEK1 and NRK1, as well as NPK1, are activated at the late M phase of the cell cycle in tobacco cells, and they are rapidly inactivated by depolymerization of phragmoplast microtubules [1].
• Thus, the catalytic domain of NPK1 specifically activates the signal transduction pathway mediated by BCK1 in yeast [2].
• The predicted amino acid sequences of the kinase domains in the amino-terminal halves of the ANPs were more than 80% identical to that of NPK1, while the kinase-unrelated regions in the carboxy-terminal halves exhibited relatively low homology [3].
• NPK1 appears to have a second role in repression of auxin-induced gene expression [4].

Biological context of KIN3

• FUN52 proved to be the same as the previously identified KIN3 gene [Jones and Rosamond, Gene 90 (1990) 87-92] that was reported to map on chromosome VI [5].
• KIN3 is unique in the yeast genome, maps to chromosome VI and is actively expressed in mitotically dividing cells to produce a 1400 nucleotide (nt) message [6].
• This gene has been temporarily designated FUN52, where FUN is the acronym for 'function unknown now'. In A. nidulans, nimA is required to enter mitosis [5].
• In contrast, S. cerevisiae cells that were either deleted for FUN52 or were overexpressing it had no detectable growth phenotypes [5].

Associations of KIN3 with chemical compounds

• Disruption of the NPK1 gene impedes neither growth on glucose or a variety of other carbon sources, nor mating or sporulation [7].

Other interactions of KIN3

• A new protein kinase gene [called NPK1 (for nonessential protein kinase)] has been found on chromosome I of Saccharomyces cerevisiae between CDC15 and ADE1 [7].

References