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Gene Review:

SEM1 - proteasome regulatory particle lid subunit...

Saccharomyces cerevisiae S288c

Synonyms: 26S proteasome complex subunit SEM1, DSH1, DSS1, HOD1, YDR363W-A

Marston, N.J. et al., Reinman, M. et al., Jäntti, J. et al., Krogan, N.J. et al., Jossé, L. et al., et al.

Reinman, Jäntti, Alfthan, Keränen, Söderlund, Takkinen,

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Disease relevance of SEM1

Deletion of SEM1 or genes encoding other nonessential 19S or 20S proteasome subunits also results in synthetic growth defects and hypersensitivity to genotoxins when combined with mutations in well-established DNA DSB repair genes [1].
Interaction between the product of the breast cancer susceptibility gene BRCA2 and DSS1, a protein functionally conserved from yeast to mammals [2].
Sem1p-specific antibody isolated from a phage display library was expressed intracellularily and targeted to either the cytosol or the nucleus of yeast cells [3].

High impact information on SEM1

This phenotype was abolished after reintroduction of either SEM1 or the mouse homologue Dss1 into the cells [4].
In diploid cells that normally do not form pseudohyphae (S288C background), deletion of SEM1 triggered pseudohyphal growth [4].
SEM1 is not an essential gene [4].
Apparent orthologues of the mammalian DSS1 gene were identified in the genome of the yeasts Schizosaccharomyces pombe and Saccharomyces cerevisiae [2].
Localization of the Fab intrabody with or without the nuclear localization signal to the nucleus in Sem1p-dependent manner suggests that Sem1p mediates the nuclear transport of the intrabody without any targeting signal [3].

Biological context of SEM1

Human DSS1 associates with BRCA2, a tumour suppressor protein required for efficient recombinational DNA repair, but the biochemical function of DSS1 is not known [5].

Analytical, diagnostic and therapeutic context of SEM1

The 19 S RP was immunoprecipitated together with Sem1 by immunoprecipitation using hemagglutinin epitope-tagged Sem1 as bait [6].
Cell fractionation showed that Sem1p is mainly cytosolic but also associates with the microsomal fraction [4].
Yeast strains in which these DSS1-like genes were deleted showed a temperature-sensitive growth phenotype, which was analyzed by flow cytometry [2].
Yeast and mammalian two-hybrid assays showed that DSS1 can associate with BRCA2 in the region of amino acids 2472 to 2957 in the C terminus of the protein [2].

References

Proteasome involvement in the repair of DNA double-strand breaks. Krogan, N.J., Lam, M.H., Fillingham, J., Keogh, M.C., Gebbia, M., Li, J., Datta, N., Cagney, G., Buratowski, S., Emili, A., Greenblatt, J.F. Mol. Cell (2004) [Pubmed]
Interaction between the product of the breast cancer susceptibility gene BRCA2 and DSS1, a protein functionally conserved from yeast to mammals. Marston, N.J., Richards, W.J., Hughes, D., Bertwistle, D., Marshall, C.J., Ashworth, A. Mol. Cell. Biol. (1999) [Pubmed]
Functional inactivation of the conserved Sem1p in yeast by intrabodies. Reinman, M., Jäntti, J., Alfthan, K., Keränen, S., Söderlund, H., Takkinen, K. Yeast (2003) [Pubmed]
SEM1, a homologue of the split hand/split foot malformation candidate gene Dss1, regulates exocytosis and pseudohyphal differentiation in yeast. Jäntti, J., Lahdenranta, J., Olkkonen, V.M., Söderlund, H., Keränen, S. Proc. Natl. Acad. Sci. U.S.A. (1999) [Pubmed]
Fission yeast Dss1 associates with the proteasome and is required for efficient ubiquitin-dependent proteolysis. Jossé, L., Harley, M.E., Pires, I.M., Hughes, D.A. Biochem. J. (2006) [Pubmed]
Sem1p is a novel subunit of the 26 S proteasome from Saccharomyces cerevisiae. Sone, T., Saeki, Y., Toh-e, A., Yokosawa, H. J. Biol. Chem. (2004) [Pubmed]

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