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Gene Review

APN2  -  DNA-(apurinic or apyrimidinic site) lyase...

Saccharomyces cerevisiae S288c

Synonyms: AP endonuclease 2, Apurinic-apyrimidinic endonuclease 2, DNA-(apurinic or apyrimidinic site) lyase 2, ETH1, YBL019W, ...
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Disease relevance of APN2


High impact information on APN2

  • Apn2 also has an AP endonuclease activity and functions in the removal of abasic sites from DNA [2].
  • Importantly, PCNA stimulates the 3'-->5' exonuclease and 3'-phosphodiesterase activities of Apn2 [2].
  • Consistent with these biochemical observations, genetic studies indicate the involvement of APN2 in the repair of H(2)O(2)-induced DNA damage in a pathway alternate to APN1, and the Ala59 mutation inactivates this function of Apn2 [3].
  • Here, we show that purified yeast Apn2 protein contains 3'-phosphodiesterase and 3'-->5' exonuclease activities, and mutation of the active-site residue Glu59 to Ala in Apn2 inactivates both these activities [3].
  • An open reading frame was found to encode a putative protein (34% identical to the Schizosaccharomyces pombe eth1(+) [open reading frame SPBC3D6.10] gene product) with a 347-residue segment homologous to the exonuclease III family of AP endonucleases [1].

Biological context of APN2


Associations of APN2 with chemical compounds

  • Synthesis of mRNA from ETH1 in wild-type cells was induced sixfold relative to that in untreated cells after exposure to the alkylating agent methyl methanesulfonate (MMS) [1].
  • Elimination of ETH1 in apn1 strains also increased spontaneous mutation rates 9- or 31-fold compared to the wild type as determined by reversion to adenine or lysine prototrophy, respectively [1].
  • This species difference was due to the absence of delta-lyase activity in S. cerevisiae, since expression of bacterial Fpg conferred Tpp1-dependent resistance to methylmethane sulfonate in yeast lacking the abasic endonucleases Apn1 and Apn2 [6].
  • While uve1 degrees cells show only a modest sensitivity to the oxidizing agent hydrogen peroxide (H(2)O(2)), both uve1 degrees and apn2 degrees cells also display a marked increased in mutation rate following exposure to H(2)O(2) doses [5].
  • Overexpression of Apn2p in nth1 mutants failed to suppress MMS sensitivity [7].

Other interactions of APN2


  1. The Saccharomyces cerevisiae ETH1 gene, an inducible homolog of exonuclease III that provides resistance to DNA-damaging agents and limits spontaneous mutagenesis. Bennett, R.A. Mol. Cell. Biol. (1999) [Pubmed]
  2. Stimulation of 3'-->5' exonuclease and 3'-phosphodiesterase activities of yeast apn2 by proliferating cell nuclear antigen. Unk, I., Haracska, L., Gomes, X.V., Burgers, P.M., Prakash, L., Prakash, S. Mol. Cell. Biol. (2002) [Pubmed]
  3. 3'-phosphodiesterase and 3'-->5' exonuclease activities of yeast Apn2 protein and requirement of these activities for repair of oxidative DNA damage. Unk, I., Haracska, L., Prakash, S., Prakash, L. Mol. Cell. Biol. (2001) [Pubmed]
  4. The role of Schizosaccharomyces pombe DNA repair enzymes Apn1p and Uve1p in the base excision repair of apurinic/apyrimidinic sites. Tanihigashi, H., Yamada, A., Igawa, E., Ikeda, S. Biochem. Biophys. Res. Commun. (2006) [Pubmed]
  5. Fission yeast Uve1 and Apn2 function in distinct oxidative damage repair pathways in vivo. Fraser, J.L., Neill, E., Davey, S. DNA Repair (Amst.) (2003) [Pubmed]
  6. The role of yeast DNA 3'-phosphatase Tpp1 and rad1/Rad10 endonuclease in processing spontaneous and induced base lesions. Karumbati, A.S., Deshpande, R.A., Jilani, A., Vance, J.R., Ramotar, D., Wilson, T.E. J. Biol. Chem. (2003) [Pubmed]
  7. Roles of base excision repair enzymes Nth1p and Apn2p from Schizosaccharomyces pombe in processing alkylation and oxidative DNA damage. Sugimoto, T., Igawa, E., Tanihigashi, H., Matsubara, M., Ide, H., Ikeda, S. DNA Repair (Amst.) (2005) [Pubmed]
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