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Gene Review

RPG1  -  Rpg1p

Saccharomyces cerevisiae S288c

Synonyms: Eukaryotic translation initiation factor 3 110 kDa subunit homolog, Eukaryotic translation initiation factor 3 subunit A, TIF32, Translation initiation factor eIF3, p110 subunit homolog, YBR0734, ...
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High impact information on RPG1

  • By characterizing the mutant subcomplexes, we confirmed all key predictions of our model and uncovered new interactions of NIP1 with PRT1 and of TIF32 with eIF1 [1].
  • In addition to the contact between eIF2 and the N-terminal domain (NTD) of NIP1 bridged by eIF5, the C-terminal domain (CTD) of TIF32 binds eIF2 directly and is required for eIF2-eIF3 association in vivo [1].
  • Overexpressing a CTD-less form of TIF32 exacerbated the initiation defect of an eIF5 mutation that weakens the NIP1-eIF5-eIF2 connection [1].
  • We propose that HCR1 stabilizes or modulates interaction between TIF32 and the PRT1 RRM [2].
  • Deletion/disruption of TIF31 results in no change in growth rate, whereas deletion of TIF32 is lethal [3].

Biological context of RPG1

  • The product of the Saccharomyces cerevisiae RPG1 gene has been described as encoding a protein required for passage through the G1 phase of the cell cycle and exhibiting significant sequence similarity to the largest subunit of human eIF3 [4].
  • The essential gene RPG1/TIF32 of Saccharomyces cerevisiae encodes the 110-kDa subunit of the translation initiation factor 3 (eIF3) core complex [5].
  • Three ORFs, YBR0726, YBR0735 and YBR0740 are completely contained in YBR0727, YBR0734 and YBR0739 respectively, and thus probably do not represent real genes [6].
  • Three probes from the distal end of the pM13 BAC mapped 3.0 cm proximal of Rpg1 and out of synteny with rice [7].
  • The barley stem rust resistance genes Rpg1 and rpg4 were mapped in barley on chromosomes 1P and 7M, respectively and the syntenous rice chromosomes identified as 6P and 3P by mapping common probes in barley and rice [7].

Anatomical context of RPG1

  • In budded cells, a portion of Rpg1p was obviously co-localised with microtubules [5].
  • We conclude that Rpg1p is a microtubule-interacting protein that indicates an interesting connection between the translation initiation machinery and cytoskeleton in yeast Saccharomyces cerevisiae [5].
  • A high-resolution map of the Rpg1 region was established with 1400 gametes yielding a map density of 3.6 markers per 0.1 cM [7].

Other interactions of RPG1

  • When overexpressed, the 30-kDa gene product, named Hcr1p, was able to support, under restrictive conditions, growth of the rpg1-1 temperature-sensitive mutant, but not of a Rpg1p-depleted mutant [8].
  • Using immunofluorescence microscopy, Rpg1p and Sla2p proteins were colocalized at the patch associated with the tip of emerging bud [9].

Analytical, diagnostic and therapeutic context of RPG1


  1. Direct eIF2-eIF3 contact in the multifactor complex is important for translation initiation in vivo. Valásek, L., Nielsen, K.H., Hinnebusch, A.G. EMBO J. (2002) [Pubmed]
  2. Related eIF3 subunits TIF32 and HCR1 interact with an RNA recognition motif in PRT1 required for eIF3 integrity and ribosome binding. Valásek, L., Phan, L., Schoenfeld, L.W., Valásková, V., Hinnebusch, A.G. EMBO J. (2001) [Pubmed]
  3. A 110-kilodalton subunit of translation initiation factor eIF3 and an associated 135-kilodalton protein are encoded by the Saccharomyces cerevisiae TIF32 and TIF31 genes. Vornlocher, H.P., Hanachi, P., Ribeiro, S., Hershey, J.W. J. Biol. Chem. (1999) [Pubmed]
  4. Rpg1, the Saccharomyces cerevisiae homologue of the largest subunit of mammalian translation initiation factor 3, is required for translational activity. Valásek, L., Trachsel, H., Hasek, J., Ruis, H. J. Biol. Chem. (1998) [Pubmed]
  5. Rpg1p, the subunit of the Saccharomyces cerevisiae eIF3 core complex, is a microtubule-interacting protein. Hasek, J., Kovarik, P., Valásek, L., Malínská, K., Schneider, J., Kohlwein, S.D., Ruis, H. Cell Motil. Cytoskeleton (2000) [Pubmed]
  6. Sequence analysis of a 31 kb DNA fragment from the right arm of Saccharomyces cerevisiae chromosome II. Van der Aart, Q.J., Barthe, C., Doignon, F., Aigle, M., Crouzet, M., Steensma, H.Y. Yeast (1994) [Pubmed]
  7. Towards map-based cloning of the barley stem rust resistance genes Rpg1 and rpg4 using rice as an intergenomic cloning vehicle. Kilian, A., Chen, J., Han, F., Steffenson, B., Kleinhofs, A. Plant Mol. Biol. (1997) [Pubmed]
  8. The Saccharomyces cerevisiae HCR1 gene encoding a homologue of the p35 subunit of human translation initiation factor 3 (eIF3) is a high copy suppressor of a temperature-sensitive mutation in the Rpg1p subunit of yeast eIF3. Valásek, L., Hasek, J., Trachsel, H., Imre, E.M., Ruis, H. J. Biol. Chem. (1999) [Pubmed]
  9. Rpg1p/Tif32p, a subunit of translation initiation factor 3, interacts with actin-associated protein Sla2p. Palecek, J., Hasek, J., Ruis, H. Biochem. Biophys. Res. Commun. (2001) [Pubmed]
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