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Gene Review

SLA2  -  Sla2p

Saccharomyces cerevisiae S288c

Synonyms: END4, MOP2, N1102, Protein SLA2, Transmembrane protein MOP2, ...
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High impact information on SLA2

  • In cells lacking the highly conserved endocytic protein Sla2p, patch motility was arrested and actin comet tails associated with endocytic patch complexes [1].
  • However, yeast mutant strains defective in endocytosis (end3, end4 and chc1-ts) and vacuolar degradation (pep4) exhibit copper-dependent Ctr1p degradation, indicating that internalization and delivery to the vacuole is not the principal mechanism responsible for degradation [2].
  • An end4 mutation, isolated for its defect in the endocytic uptake of alpha-factor pheromone (Raths, S., J. Rohrer, F. Crausaz, and H. Riezman. 1993. J. Cell Biol. 120:55-65), blocks constitutive endocytosis of the a-factor receptor, yet fails to block ubiquitination of the receptor [3].
  • Null mutations in SLA1 and SLA2 cause temperature-sensitive growth defects [4].
  • In addition, synthetic-lethal interactions were observed for double-mutants containing null alleles of SLA2 and SAC6 [4].

Biological context of SLA2


Anatomical context of SLA2


Associations of SLA2 with chemical compounds

  • A liposome-binding assay revealed that Sla2p binds to PtdIns(4,5)P2 specifically through its ANTH domain and identified specific lysine residues required for this interaction [11].
  • Ligand-mediated repression of spermidine transport was delayed in end3 and end4 mutants that are deficient in the initial steps of the endocytic pathway, and spermidine uptake activity was increased 4- to 5-fold in end3 mutants relative to parental cells, although the stability of the transport system was similar in both strains [12].
  • Generation of an Deltasla1Deltasla2 double mutant demonstrates that Sla2p is likely to act upstream of Sla1p in endocytosis, whereas sensitivity to latrunculin-A suggests that the proteins have opposite effects on actin dynamics [13].

Physical interactions of SLA2


Regulatory relationships of SLA2


Other interactions of SLA2

  • Mutant alleles of three genes, DOA4, SLA1 and SLA2, were recovered [5].
  • (iii) Rsp5p and Sla2p coimmunoprecipitate and partially colocalize to punctate structures in wild-type cells [15].
  • Negative Regulation of Yeast Eps15-like Arp2/3 Complex Activator, Pan1p, by the Hip1R-related Protein, Sla2p, during Endocytosis [14].
  • Modifier of pma1 (mop) mutants were isolated as enhancers of the mutant phenotypes of pma1 mutants. mop2 mutations reduce the abundance and activity of Pma1 protein on the plasma membrane without affecting the abundance of other prominent plasma membrane proteins [9].
  • Patches that do internalize receive Sla1p late, which is followed by Abp1, which appears near the end of Sla2p lifetime [16].

Analytical, diagnostic and therapeutic context of SLA2


  1. A pathway for association of receptors, adaptors, and actin during endocytic internalization. Kaksonen, M., Sun, Y., Drubin, D.G. Cell (2003) [Pubmed]
  2. Copper-dependent degradation of the Saccharomyces cerevisiae plasma membrane copper transporter Ctr1p in the apparent absence of endocytosis. Ooi, C.E., Rabinovich, E., Dancis, A., Bonifacino, J.S., Klausner, R.D. EMBO J. (1996) [Pubmed]
  3. Ubiquitination of the yeast a-factor receptor. Roth, A.F., Davis, N.G. J. Cell Biol. (1996) [Pubmed]
  4. Synthetic-lethal interactions identify two novel genes, SLA1 and SLA2, that control membrane cytoskeleton assembly in Saccharomyces cerevisiae. Holtzman, D.A., Yang, S., Drubin, D.G. J. Cell Biol. (1993) [Pubmed]
  5. The deubiquitinating enzyme Doa4p protects cells from DNA topoisomerase I poisons. Fiorani, P., Reid, R.J., Schepis, A., Jacquiau, H.R., Guo, H., Thimmaiah, P., Benedetti, P., Bjornsti, M.A. J. Biol. Chem. (2004) [Pubmed]
  6. End4p/Sla2p interacts with actin-associated proteins for endocytosis in Saccharomyces cerevisiae. Wesp, A., Hicke, L., Palecek, J., Lombardi, R., Aust, T., Munn, A.L., Riezman, H. Mol. Biol. Cell (1997) [Pubmed]
  7. Temperature-sensitive mutations in the Saccharomyces cerevisiae MRT4, GRC5, SLA2 and THS1 genes result in defects in mRNA turnover. Zuk, D., Belk, J.P., Jacobson, A. Genetics (1999) [Pubmed]
  8. Sequence analysis of SLA2 of the dimorphic yeasts Candida albicans and Yarrowia lipolytica. Melms, A.S., Gausmann, U., Swoboda, R.K., Dominguez, A., Kurischko, C. Yeast (1999) [Pubmed]
  9. MOP2 (SLA2) affects the abundance of the plasma membrane H(+)-ATPase of Saccharomyces cerevisiae. Na, S., Hincapie, M., McCusker, J.H., Haber, J.E. J. Biol. Chem. (1995) [Pubmed]
  10. Yeast actin cytoskeleton mutants accumulate a new class of Golgi-derived secretary vesicle. Mulholland, J., Wesp, A., Riezman, H., Botstein, D. Mol. Biol. Cell (1997) [Pubmed]
  11. Interaction of Sla2p's ANTH domain with PtdIns(4,5)P2 is important for actin-dependent endocytic internalization. Sun, Y., Kaksonen, M., Madden, D.T., Schekman, R., Drubin, D.G. Mol. Biol. Cell (2005) [Pubmed]
  12. The spermidine transport system is regulated by ligand inactivation, endocytosis, and by the Npr1p Ser/Thr protein kinase in Saccharomyces cerevisiae. Kaouass, M., Gamache, I., Ramotar, D., Audette, M., Poulin, R. J. Biol. Chem. (1998) [Pubmed]
  13. An interaction between Sla1p and Sla2p plays a role in regulating actin dynamics and endocytosis in budding yeast. Gourlay, C.W., Dewar, H., Warren, D.T., Costa, R., Satish, N., Ayscough, K.R. J. Cell. Sci. (2003) [Pubmed]
  14. Negative Regulation of Yeast Eps15-like Arp2/3 Complex Activator, Pan1p, by the Hip1R-related Protein, Sla2p, during Endocytosis. Toshima, J., Toshima, J.Y., Duncan, M.C., Cope, M.J., Sun, Y., Martin, A.C., Anderson, S., Yates, J.R., Mizuno, K., Drubin, D.G. Mol. Biol. Cell (2007) [Pubmed]
  15. Rsp5p, a new link between the actin cytoskeleton and endocytosis in the yeast Saccharomyces cerevisiae. Kamińska, J., Gajewska, B., Hopper, A.K., Zoładek, T. Mol. Cell. Biol. (2002) [Pubmed]
  16. Clathrin is Important for Normal Actin Dynamics and Progression of Sla2p-Containing Patches During Endocytosis in Yeast. Newpher, T.M., Lemmon, S.K. Traffic (2006) [Pubmed]
  17. An actin-binding protein of the Sla2/Huntingtin interacting protein 1 family is a novel component of clathrin-coated pits and vesicles. Engqvist-Goldstein, A.E., Kessels, M.M., Chopra, V.S., Hayden, M.R., Drubin, D.G. J. Cell Biol. (1999) [Pubmed]
  18. Rpg1p/Tif32p, a subunit of translation initiation factor 3, interacts with actin-associated protein Sla2p. Palecek, J., Hasek, J., Ruis, H. Biochem. Biophys. Res. Commun. (2001) [Pubmed]
  19. Novel function of clathrin light chain in promoting endocytic vesicle formation. Newpher, T.M., Idrissi, F.Z., Geli, M.I., Lemmon, S.K. Mol. Biol. Cell (2006) [Pubmed]
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