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Gene Review

MED8  -  Med8p

Saccharomyces cerevisiae S288c

Synonyms: Mediator complex subunit 8, Mediator of RNA polymerase II transcription subunit 8, YBR1403, YBR193C
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Disease relevance of MED8

  • To determine whether the p27 protein represents the Med8 product, we expressed MED8 gene in E. coli and demonstrated that the heterologous synthesized protein specifically binds to both UASSUC2 and DRS2HXK2 [1].

High impact information on MED8

  • As part of our effort to understand the functions of Elongin BC-based ubiquitin ligases, we exploited a modified yeast two-hybrid screen to identify a mammalian BC-box protein similar in sequence to Saccharomyces cerevisiae Mediator subunit Med8p [2].
  • Taken together, these results suggest that assembly of Mediator is a multistep process that involves conversion of both Med4 and Med8 to their low mobility forms [3].
  • Because this site was previously involved in the Hxk2p-dependent glucose-induced regulation of gene transcription, we tested whether Hxk2p interacts with Med8p [4].
  • Mediator factor Med8p interacts with the hexokinase 2: implication in the glucose signalling pathway of Saccharomyces cerevisiae [4].
  • The amino terminal sequence of p27 identified the MED8 gene (open reading frame YBR193C), located in chromosome II of S. cerevisiae, as the gene coding for the protein [1].

Biological context of MED8


Other interactions of MED8


  1. Med8, a subunit of the mediator CTD complex of RNA polymerase II, directly binds to regulatory elements of SUC2 and HXK2 genes. Chaves, R.S., Herrero, P., Moreno, F. Biochem. Biophys. Res. Commun. (1999) [Pubmed]
  2. Mammalian mediator subunit mMED8 is an Elongin BC-interacting protein that can assemble with Cul2 and Rbx1 to reconstitute a ubiquitin ligase. Brower, C.S., Sato, S., Tomomori-Sato, C., Kamura, T., Pause, A., Stearman, R., Klausner, R.D., Malik, S., Lane, W.S., Sorokina, I., Roeder, R.G., Conaway, J.W., Conaway, R.C. Proc. Natl. Acad. Sci. U.S.A. (2002) [Pubmed]
  3. Functional interactions within yeast mediator and evidence of differential subunit modifications. Balciunas, D., Hallberg, M., Björklund, S., Ronne, H. J. Biol. Chem. (2003) [Pubmed]
  4. Mediator factor Med8p interacts with the hexokinase 2: implication in the glucose signalling pathway of Saccharomyces cerevisiae. de la Cera, T., Herrero, P., Moreno-Herrero, F., Chaves, R.S., Moreno, F. J. Mol. Biol. (2002) [Pubmed]
  5. Analysis by atomic force microscopy of Med8 binding to cis-acting regulatory elements of the SUC2 and HXK2 genes of saccharomyces cerevisiae. Moreno-Herrero, F., Herrero, P., Colchero, J., Baró, A.M., Moreno, F. FEBS Lett. (1999) [Pubmed]
  6. RIM2, MSI1 and PGI1 are located within an 8 kb segment of Saccharomyces cerevisiae chromosome II, which also contains the putative ribosomal gene L21 and a new putative essential gene with a leucine zipper motif. Démolis, N., Mallet, L., Bussereau, F., Jacquet, M. Yeast (1993) [Pubmed]
  7. The Schizosaccharomyces pombe sep15+ gene encodes a protein homologous to the Med8 subunit of the Saccharomyces cerevisiae transcriptional mediator complex. Zilahi, E., Miklós, I., Sipiczki, M. Curr. Genet. (2000) [Pubmed]
  8. Tpk3 and Snf1 protein kinases regulate Rgt1 association with Saccharomyces cerevisiae HXK2 promoter. Palomino, A., Herrero, P., Moreno, F. Nucleic Acids Res. (2006) [Pubmed]
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